Use of the 5-cyano-2,3-ditolyl tetrazolium chloride reduction test to assess respiring marine bacteria and grazing effects by flow cytometry during linear alkylbenzene sulfonate degradation

Abstract Viable, total and metabolically active bacteria were determined during linear alkylbenzene sulfonate degradation in coastal seawater. Viable bacteria were estimated by plate counts on marine agar media while the total and metabolically active bacteria were determined with the nucleic acid s...

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Bibliographic Details
Published in:FEMS microbiology ecology 1998-09, Vol.27 (1), p.33-42
Main Authors: López-Amorós, Ricard, Comas, Jaume, García, María Teresa, Vives-Rego, Josep
Format: Article
Language:English
Subjects:
5‐Cyano‐2,3‐ditolyl tetrazolium chloride
Alkylbenzene sulfonate
Alkylbenzenesulfonates
Bacteria
Bacterioplankton
Biodegradation
Chemical analysis
Chlorides
Degradation
Detergent industry
Ecology
Flow cytometry
Grazing
High performance liquid chromatography
Linear alkylbenzene sulfonate
Liquid chromatography
Marine bacteria
Microbiology
Nucleic acids
Protozoa
Raw materials
Seawater
Sodium linear alkylbenzene sulfonate
SYTO‐13
Tetrazolium salt
Water analysis
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Summary:Abstract Viable, total and metabolically active bacteria were determined during linear alkylbenzene sulfonate degradation in coastal seawater. Viable bacteria were estimated by plate counts on marine agar media while the total and metabolically active bacteria were determined with the nucleic acid stain SYTO-13 and the tetrazolium salt 5-cyano-2,3-ditolyl tetrazolium chloride, respectively, in double stain procedures analyzed by flow cytometry. The double stain SYTO-13/5-cyano-2,3-ditolyl tetrazolium chloride is a rapid and simple method that discriminates bacterioplankton populations according to nucleic acid content and formazan formation. Linear alkylbenzene sulfonate degradation was monitored by high-performance liquid chromatography analysis. Bacterioplankton degraded linear alkylbenzene sulfonate by growing to communities with a high percentage of viable and metabolically active bacteria. The bacteria produced were rapidly grazed by protozoa; however, the grazing took place mostly on metabolically active cells, which were larger than the rest of the population.
ISSN:0168-6496
1574-6941
DOI:10.1111/j.1574-6941.1998.tb00523.x