Heat curing of UTMA-based hybrid resin: effects on the degree of conversion and cytotoxicity

• Published in: Odontology 2004-09, Vol.92 (1), p.27-35
• Main Authors: S ilynoja, Eija S., Shinya, Akiyoshi, Koskinen, Mika K., I. Salonen, Jukka, Masuda, Takashi, Shinya, Akikazu, Matsuda, Tetsuji, Mihara, Takashi, Koide, Naoyuki
• Format: Article
• Language: English
• Subjects: Carbon - chemistry; Cells, Cultured; Chemical Phenomena; Chemistry, Physical; Composite Resins - chemistry; Composite Resins - radiation effects; Composite Resins - toxicity; Culture Media; Dental research; Fibroblasts - drug effects; Gingiva - cytology; Gingiva - drug effects; Glass - chemistry; Glass - radiation effects; Hot Temperature; Humans; Hydrogen - chemistry; Light; Materials Testing; Methacrylates - chemistry; Methacrylates - radiation effects; Methacrylates - toxicity; Microscopy, Electron, Scanning; Nitrogen - chemistry; Polyurethanes - chemistry; Polyurethanes - radiation effects; Polyurethanes - toxicity; Porosity; Spectroscopy, Fourier Transform Infrared; Surface Properties; Time Factors
• ISSN: 1618-1247; 1618-1255
• DOI: 10.1007/s10266-004-0037-2

This study was designed to determine the effects of the heat curing time on a urethane tetramethacrylate (UTMA)-based hybrid resin and specifically on the degree of conversion (DC) and cytotoxicity. The materials used in this study were Estenia, a new-generation hybrid resin, and an experimental fiber reinforcement, Br-100. The DC values of the hybrid resin samples were measured using a Fourier transform infrared (FTIR) spectrophotometer after 180s of light curing followed by heat curing (0, 15, 30, and 60min). A method comparing intensities of C=C and N-H vibrations of the sample was used to calculate the final DC values. FTIR spectra were measured both inside and on the surface of the sample. The calculated DC values increased by increasing the heat curing times. After light curing only and after 15-min heat curing, the DC values inside the samples were smaller than the corresponding DC values at the surfaces of the samples. After 60min of heat curing, the samples achieved homogeneous polymerization (DC% = 65). The cytotoxicity of the material was studied from the glass fiber-reinforced hybrid resin samples, which were first light cured and then heat cured (15, 30, and 60min). Cytotoxicity was tested using both direct contact and extract methods. For the extract tests, the test specimens were incubated in a cell culture media at 370, 540 or 72°C for 24h. The heat curing times used had no effect on cytotoxicity. The incubation temperature, however, did have a significant effect. The extract obtained from 72°C incubation showed a cytotoxic effect whereas the others did not. The direct contact test did not show cytotoxicity.