lncrps25 play an essential role in motor neuron development through controlling the expression of olig2 in zebrafish

• Published in: Journal of cellular physiology 2020-04, Vol.235 (4), p.3485-3496
• Main Authors: Gao, Tianheng, Li, Jingyun, Li, Nan, Gao, Yan, Yu, Lingling, Zhuang, Sisi, Zhao, Yingmin, Dong, Xiaohua
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Animals; Cell Differentiation - genetics; Conserved sequence; Danio rerio; development; Embryogenesis; Embryonic Development - genetics; Embryos; Gene expression; Gene Expression Regulation, Developmental - genetics; Gene sequencing; Humans; lncrps25; Morpholinos - genetics; motor neuron; Motor neurons; Motor Neurons - metabolism; Neurogenesis - genetics; Neurons; olig2; Olig2 protein; Oligodendrocyte Transcription Factor 2 - genetics; Polymerase chain reaction; Proteins; Regeneration; Ribonucleic acid; Ribosomal Proteins - genetics; RNA; RNA, Long Noncoding - genetics; Spinal cord; Spinal Cord - growth & development; Spinal Cord - metabolism; Swimming; Zebrafish; Zebrafish - genetics; Zebrafish - growth & development; Zebrafish Proteins - genetics
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.29237

lncrps25 is an intergenic long noncoding RNA (lncRNA), which is location close to rps25 (ribosomal protein S25) gene, is reported share high conserved sequence with NREP (neuronal regeneration‐related protein) 3′‐untranslated region. The function and mechanism of most of the lncRNA in embryo development remain largely unknown. In zebrafish, lncrps25 is widely expressed in the early embryonic stage and spinal cord during development. Morpholino (MO) knockdown of zebrafish lncrps25 exhibit locomotor behavior defects, caused by abnormal development of motor neurons. In addition, the defect of swimming ability and motor neurons could be recovery by microinject with lncrps25 RNA in lncrps25 morphants. By performing RNA sequencing and quantitative real‐time polymerase chain reaction, we found that olig2 (oligodendrocyte transcription factor 2) messenger RNA (mRNA) was downregulated in lncrps25 morphants. Moreover, overexpression of olig2 mRNA in lncrps25 morphants partially rescued motor neurons development. Taken together, these results indicate that lncrps25 plays an essential role in the development of motor neurons in zebrafish. Our studies have demonstrated that long noncoding RNA lncrps25 is required for motor neuron development and axonal growth through regulating the expression of olig2.