Effect of Aromatase Inhibition (Exemestane) on Urine Concentration of Osteoprotegerin in Healthy Postmenopausal Women

• Published in: Journal of clinical pharmacology 2020-02, Vol.60 (2), p.209-217
• Main Authors: Garcia, Allison P., Hatvany, Jacob B., Murphy, Michael A., Atchley, Daniel H., Gurley, Bill J., Kamdem, Landry K.
• Format: Article
• Language: English
• Subjects: Aged; Androstadienes - administration & dosage; Androstadienes - pharmacokinetics; Androstadienes - pharmacology; Androstadienes - urine; Aromatase; aromatase inhibitors; Aromatase Inhibitors - administration & dosage; Aromatase Inhibitors - pharmacokinetics; Aromatase Inhibitors - pharmacology; Aromatase Inhibitors - urine; Breast cancer; Data processing; Dosage; exemestane; Female; Healthy Volunteers; Hormone replacement therapy; Humans; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Menopause; Middle Aged; Osteoprotegerin; Osteoprotegerin - urine; Pharmacodynamics; Pharmacokinetics; Pilot Projects; Plasma; Post-menopause; Postmenopause; Rank tests; Retrospective Studies; Urine
• ISSN: 0091-2700; 1552-4604
• DOI: 10.1002/jcph.1519

This pilot study examined how exemestane (an aromatase inhibitor [AI]) affected osteoprotegerin (OPG) urine concentrations in postmenopausal women. Exemestane (25 mg, single dose) was given to 14 disease‐free women past menopause in this nonrandomized, open‐label study. Before dosing, urine specimens were gathered. Three days later, these women returned to provide urine specimens for pharmacokinetic (measurement of major parent drug and enzymatic product) and pharmacodynamic (profiling of OPG) analysis. Urine concentrations of the major parent drug (exemestane) and enzymatic product (17‐hydroexemestane) were quantified using liquid chromatography‐tandem mass spectrometry. An analyst software package was used for data processing. Following the manufacturer's guidelines, OPG urine concentrations were quantified using a human osteoprotegerin TNFRSF11b ELISA kit from Sigma‐Aldrich. A microplate reader helped to carry out OPG data analysis and processing. Our results highlight that OPG urine concentrations were decreased 3 days after drug dosage (mean predosage OPG concentration, 61.4 ± 24.1 pg/mL; vs mean postdosage OPG concentration, 45.7 ± 22.1 pg/mL; P = .02, Wilcoxon rank test). Among the 14 volunteers enrolled in the study, 4 subjects had an increase of less than 1‐fold, and the rest showed an average of a 2‐fold decrease in OPG concentration (range, 1.1‐5.4; standard deviation, 1.3) after exemestane administration. There was no association between fold decrease in OPG urine concentration and the pharmacokinetics of the major parent drug (exemestane) and its enzymatic product (17‐hydroexemestane). We concluded that one of the off‐target pharmacological effects of AIs (eg ,exemestane) may result in the reduction of osteoprotegerin.