Absorption spectrum analysis of dentine sialophosphoprotein (DSPP) in orthodontic patient

• Main Authors: Zain, M. N. M., Yusof, Z. M., Yazid, F., Ashari, A., Wong, K. S. H., Lee, W. J., Tan, K. F., Ariffin, S. H. Z., Wahab, R. M. A.
• Format: Conference Proceeding
• Language: English
• Subjects: Absorption spectra; Biomarkers; Data models; Model accuracy; Orthodontics; Proteins; Qualitative analysis; Spectrum analysis; Teeth
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/1.5142099

A force applied during the orthodontic treatment induces inflammation which is essential for tooth movement, can lead to root resorption. Previous research works have proved a protein biomarker which can monitor the root resorption during orthodontic tooth movement. Dentine sialophosphoprotein (DSPP) is one of most abundant non- collagenous protein in dentine. DSPP is released into Gingival crevicular fluid (GCF) during external root resorption. In this work, we investigate and analysis the absorbance spectrum of human DSPP by using spectroscopy and a qualitative model using Soft Independent Modelling Class Analogies (SIMCA). The absorption spectrum data will be used as an indicator for clinical examination of root resorption in orthodontic treatment. The subjects for this study consisted of non- orthodontic and orthodontic patients based on different clinical treatment period. GCF samples collected from both non- orthodontic and orthodontic groups showed ultra-violet spectrum range from 244.11 to 259.86 nm. The spectrum data model accuracy for non-orthodontic and orthodontic patient obtained at 0.91. The result indicates that GCF absorption spectrums obtained correlated with the duration of orthodontic treatment after the occurrence of Orthodontic-induced inflammatory root resorption (OIIRR). The qualitative spectrum data model developed is capable to classify samples into non-orthodontic and orthodontic groups.