Oxidative damage in forage rape (Brassica napus L.) seeds following heat stress during seed development

A short period (240°C hr; Tb = 25°C) of heat stress (30°C day/25°C night) during forage rape (Brassica napus L.) seed development or at seed physiological maturity can reduce seed vigour, but the extent of oxidative damage associated with this short heat stress was not known. Heat‐stressed seeds wer...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agronomy and crop science (1986) 2020-02, Vol.206 (1), p.101-117
Main Authors: Rashid, Muhammad, Hampton, John G., Shaw, Martin L., Rolston, M. Philip, Khan, Khan Mir, Saville, Dave J.
Format: Article
Language:English
Subjects:
Accumulation
Adenosine
antioxidant enzymes
Antioxidants
Brassica
Brassica napus
Cell walls
Damage
Energy charge
Enzymatic activity
Enzyme activity
Enzymes
Heat stress
Heat tolerance
Hydrogen peroxide
Image transmission
Lipid bodies
Lipid peroxidation
Lipids
Malondialdehyde
Maturity
Membranes
metabolic activities
Mitochondria
nucleotides
Peroxidation
Physiology
Plasma membranes
Rape
Rape plants
reactive oxygen species
seed ultrastructure
seed vigour
Seeds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A short period (240°C hr; Tb = 25°C) of heat stress (30°C day/25°C night) during forage rape (Brassica napus L.) seed development or at seed physiological maturity can reduce seed vigour, but the extent of oxidative damage associated with this short heat stress was not known. Heat‐stressed seeds were assessed for malondialdehyde (MDA) content, hydrogen peroxide (H2O2) accumulation, antioxidant enzyme activity, adenylate energy charge and seed ultrastructural integrity, and compared with that of non‐heat‐stressed seeds. Heat stress increased both MDA content and H2O2 accumulation by 35%–50%, reduced antioxidant enzyme activity by between 12% and 67%, and significantly reduced adenosine energy charge. Transmission electron microscope images showed clear evidence of seed deterioration in heat‐stressed seeds, including ruptures in cell wall and plasma membranes, fused lipid bodies and damaged mitochondria. Heat stress at physiological maturity caused more oxidative damage than the same heat stress during seed development. Seed vigour decreased as H2O2 accumulation increased and antioxidant enzyme activity decreased, but no direct relationship between lipid peroxidation and seed vigour was established. The extent of damage resulting from even shorter periods of heat stress (
ISSN:0931-2250
1439-037X
DOI:10.1111/jac.12372