Effect of different homogenization pressure on soy protein isolate-vitamin D3 complex

• Published in: Process biochemistry (1991) 2019-12, Vol.87, p.145-150
• Main Authors: Zhang, Anqi, Chen, Shuang, Wang, Yuying, Zhou, Guowei, Wang, Lin, Wang, Xibo, Xu, Ning
• Format: Article
• Language: English
• Subjects: Coils; Embedding; Fluorescence; High pressure; Homogenization; Homogenization pressure; Hydrophobicity; Irradiation; Load distribution; Loading rate; Nanocomposite; Nanocomposites; Particle size; Pressure; Pressure effects; Proteins; Random coil; Soy protein isolate; Ultraviolet radiation; Vitamin D3
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2019.09.011

[Display omitted] •Homogenization changed the binding rate of soy protein isolate and vitamin D3.•The embedding rate was the highest when the pressure was 100 Mpa.•The properties of soy protein isolate - vitamin D3 complex were improved. Soy protein isolate (SPI) and vitamin D3 (VD3) interaction may be a way to improve the stability of VD3. In this paper, SPI-VD3 nanocomposites were prepared by using soy protein isolate and VD3 under different homogenization pressure conditions to study the changes of SPI structure and VD3 stability in the complex. When the homogenization pressure was 100 MPa, the encapsulation efficiency, loading rate and average particle size of the SPI-VD3 composite were the best, and the embedding rate and load ratio of the prepared SPI-VD3 nanocomposite after high pressure homogenization nanocomposites were higher, 79.21% and 7.92%, respectively. The average particle size is small, the particle distribution is relatively uniform, and the average particle size is 82.00 nm. The photo stability of the SPI-VD3 nanocomposite after high pressure homogenization prepared under this process was also significantly improved. Compared with pure VD3, the residual rate of VD3 increased from 18% to 48% after 4 h of irradiation under UV light. Under appropriate homogenization conditions, the hydrophobicity of SPI was gradually increased, and the content of α-helix and random coil of SPI-VD3 nanocomposite after high pressure homogenization was increased, and the fluorescence intensity was also gradually enhanced.