Transcriptome assembly and polymorphism detection in Silene ciliata (Caryophyllaceae)

Silene ciliata (Caryophyllaceae) is a key species to test evolutionary hypotheses in a global warming context. The recent advances in Next Generation Sequencing technologies can help in providing clues about climate-mediated local adaptation. In the present study, we analysed the full transcriptome...

Full description

Saved in:
Bibliographic Details
Published in:Plant genetic resources: characterization and utilization 2019-10, Vol.17 (5), p.452-455
Main Authors: Sacristán-Bajo, Sandra, García-Fernández, Alfredo, Iriondo, Jose M., Lara-Romero, Carlos
Format: Article
Language:English
Subjects:
Adaptation
Biological evolution
Caryophyllaceae
Climate change
Endangered & extinct species
Environmental conditions
Gene expression
Genes
Genetic diversity
Genomes
Genomics
Global warming
Next-generation sequencing
Nucleotides
Ontology
Polymorphism
Sequences
Short Communication
Silene ciliata
Single-nucleotide polymorphism
Species
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Silene ciliata (Caryophyllaceae) is a key species to test evolutionary hypotheses in a global warming context. The recent advances in Next Generation Sequencing technologies can help in providing clues about climate-mediated local adaptation. In the present study, we analysed the full transcriptome of six individuals of S. ciliata from Central Spain, by aligning it with the transcriptome of S. latifolia. We aimed (a) to identify Single Nucleotide Polymorphisms (SNPs) in the transcriptome of the species, (b) to describe the biological function of the polymorphic genes expressed and (c) to identify loci that may be involved in local adaptation processes at optimal and marginal populations of the species. We identified a total of 147,118 SNPs distributed throughout 12,688 sequences. The number of polymorphic sequences annotated was 8023. One hundred thirty sequences containing polymorphisms strongly associated with optimal and marginal conditions were selected. Gene ontology searches were successful for 118, and many of these were related to responses to stress (n = 19) and abiotic stimulus (n = 16). Genomic data generated provide a starting point for further research on the identification of candidate genes related to local adaptation and other processes in the species.
ISSN:1479-2621
1479-263X
DOI:10.1017/S1479262119000157