Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition

Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bact...

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Bibliographic Details
Published in:International journal of dairy technology 2020-02, Vol.73 (1), p.76-87
Main Authors: Ale, Elisa C., Batistela, Virginia A., Correa Olivar, Gabriela, Ferrado, Joana B., Sadiq, Sohaib, Ahmed, Hafiz I., Reinheimer, Jorge A., Vera‐Candioti, Luciana, Laws, Andrew P., Binetti, Ana Griselda
Format: Article
Language:English
Subjects:
Ammonium
Bacteria
Chemical composition
Citric acid
Dairy products
Exopolysaccharides
Glucan
Lactic acid
Lactic acid bacteria
Lactobacillus fermentum
Optimization
Organic chemistry
Response surface methodology
Statistical optimisation
Sucrose
Sugar
Yeasts
β-Glucan
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Summary:Lactobacillus fermentum Lf2 produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functional and technological roles when applied as a food ingredient in dairy matrices, properties that made these EPS interesting in comparison with other similar molecules from lactic acid bacteria (LAB). Those characteristics encouraged us to optimise the production. The EPS extract is composed of a high molecular mass β‐glucan and a medium molecular mass heteroglycan. In the present work, the optimal conditions that doubled the EPS yield using a semidefined medium (SDM, 0.63% yeast nitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH 6.5) were found by means of response surface methodology (RSM). The chemical characterisation indicated that under optimised conditions the synthesis of the heteroglycan was favoured compared with that of the β‐glucan.
ISSN:1364-727X
1471-0307
DOI:10.1111/1471-0307.12639