Microbial diversity of Livanjski cheese with the emphasis on lactic acid bacteria based on culture‐dependent and sequencing method

The microbial diversity of artisanal Livanjski cheese from traditional farms and dairies was analysed. The polyphasic approach included the isolation of microorganisms by using culture‐dependent methods and the identification of viable isolates by sequencing methods. In total, 159 isolates were iden...

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Bibliographic Details
Published in:International journal of dairy technology 2020-02, Vol.73 (1), p.202-214
Main Authors: Vladimír, Dráb, Miloslava, Kavková, Markéta, Markvartová, Jaroslava, Hanáková, Petr, Roubal
Format: Article
Language:English
Subjects:
Bacteria
Cheese
Dairies
Dairy products
Farms
Fungi
Hygiene
Identification methods
Lactic acid
Lactic acid bacteria
Livanjski cheese
Microbial diversity
Microorganisms
Sequencing
Strains (organisms)
Yeasts
Yogurt
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Summary:The microbial diversity of artisanal Livanjski cheese from traditional farms and dairies was analysed. The polyphasic approach included the isolation of microorganisms by using culture‐dependent methods and the identification of viable isolates by sequencing methods. In total, 159 isolates were identified, including five strains of yeast and 15 strains of fungi. Twenty‐two species of homofermentative and heterofermentative lactic acid bacteria were classified. The Shannon–Wiener index of diversity ranged from 0.462 to 2.51 within the cheese samples from the farms and dairies. The hygiene conditions during cheese production and the age of the cheese significantly influenced the counts and diversity of microorganisms.
ISSN:1364-727X
1471-0307
DOI:10.1111/1471-0307.12638