Determination of cancer-associated fibroblast and stromal phenotypes as novel prognostic factors for colorectal carcinomas associated with tumor budding

Objective: Tumor microenvironments consisting of stroma and extracellular matrix play important roles in tumor progression, particularly through the induction of epithelial-mesenchymal transition (EMT). It has been suggested that the phenotype of cancer-associated fibroblasts (CAFs) in stroma might...

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Bibliographic Details
Published in:Journal of natural science, biology and medicine biology and medicine, 2019-11, Vol.10 (3), p.68-72
Main Authors: Hilbertina, Noza, Siregar, Nurjati, Abdullah, Murdani, Wanandi, Septelia
Format: Article
Language:English
Subjects:
Cancer
Collagen
Colorectal cancer
Colorectal carcinoma
Extracellular matrix
Fibroblasts
Genotype & phenotype
Lymph nodes
Lymphatic system
Medical prognosis
Mesenchyme
Metastases
Metastasis
Microenvironments
Morphology
Paraffin
Pathology
Patients
Phenotypes
Prognosis
Smooth muscle
Stroma
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Summary:Objective: Tumor microenvironments consisting of stroma and extracellular matrix play important roles in tumor progression, particularly through the induction of epithelial-mesenchymal transition (EMT). It has been suggested that the phenotype of cancer-associated fibroblasts (CAFs) in stroma might be correlated with the prognosis of patients with colorectal carcinoma (CRC). We aimed to determine the stromal and CAF types for prognostic determinants of CRCs associated with tumor budding (TB) grade, reflecting EMT. Materials and Methods: Using hematoxylin and eosin-stained paraffin wax sections from 23 patients with CRC, three stromal and two CAF phenotypes were evaluated, TB grades, invasion depth, lymph node metastases, and lymphovascular invasion (LVI) were also analyzed as established prognostic determinants. Data were analyzed statistically using the Chi-squared tests. Results: There was a significant association between CAF phenotype and TB grade (P < 0.01). CRC specimens with immature CAF have higher TB grades than the mature phenotype. Nevertheless, a significant association between stroma and TB grade could not be demonstrated. Moreover, high TB grades were significantly associated with lymph node metastasis (P < 0.01) and LVI (P < 0.01). However, there were no significant associations between CAF phenotype and either of these prognostic determinants. Conclusion: CAF phenotype could be considered as a prognostic determinant of CRC through its association with TB grade, indicating the role of CAFs in EMT processes. Future studies are required to examine the secretomes of CAFs that play important roles in EMT and determine the prognosis for patients with CRCs.
ISSN:0976-9668
2229-7707
DOI:10.4103/jnsbm.JNSBM_61_19