Characterization and diagnostic marker development for Yr28-rga1 conferring stripe rust resistance in wheat

Stripe rust is one of the most devastating wheat diseases and developing resistant cultivars is the most economical way to manage this disease. More than 80 stripe rust resistance genes ( Yr genes) have formally been mapped. However, a lack of diagnostic markers has partially restricted the deployme...

Full description

Saved in:
Bibliographic Details
Published in:European journal of plant pathology 2020-02, Vol.156 (2), p.623-634
Main Authors: Zheng, Shigang, Wu, Yu, Zhou, Min, Zeng, Lian, Liu, Rong, Li, Yunfang, Liu, Zehou, Zhang, Chihong, Lu, Lu, Zhang, Lei
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Cultivars
Diagnostic systems
Disease control
Ecology
Gene sequencing
Genes
Germplasm
High temperature
Life Sciences
Losses
Marker-assisted selection
Markers
NBS-LRR gene
NBS-LRR protein
Plant breeding
Plant Pathology
Plant resistance
Plant Sciences
Protein structure
Proteins
Stripe rust
Wheat
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Stripe rust is one of the most devastating wheat diseases and developing resistant cultivars is the most economical way to manage this disease. More than 80 stripe rust resistance genes ( Yr genes) have formally been mapped. However, a lack of diagnostic markers has partially restricted the deployment of some resistance genes in molecular breeding. Among them, Yr28 showed high-temperature adult plant resistance (HT-APR) to stripe rust in the field, but it is rarely employed in wheat breeding. Here, Yr28-rga1 was found and isolated through the co-segregated marker mwg634 of Yr28 . The gene sequence of Yr28-rga1 - 4A and Yr28-rga1 - 4B were almost the same between PI648511 and other wheat germplasm, including Chinese Spring (CS), Avocet, Yanzhan1, and Chuanyu12, but Yr28-rga1 - 4D showed only 88% identity between PI648511 and the others. Further, the high similarity in conserved domains to other wheat R proteins indicated that Yr28-RGA1 was a typical NBS-LRR protein. But the low identity in amino sequences also suggested that it was a novel gene. The specific 3-D protein structure in LRR regions also indicated that Yr28-rga1 - 4D from PI648511 might have different functional responses to disease. Furthermore, two gene-specific markers of cib-Yr28M1 and cib-Yr28M2 were developed for Yr28-rga1 . Only 24 accessions showed identity to PI648511 on Yr28-rga1 locus in a panel of 672 wheat germplasms. The two diagnostic markers also revealed the positive correlation of Yr28-rga1 and stripe rust resistance (R 2  > 0.8; p  
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-019-01912-x