Effects of stressful physico-chemical factors on the fitness of the plant pathogenic bacterium Dickeya solani

Dickeya solani is a pectinolytic bacterium that causes significant losses of potato crops. Interaction between bacteria and plant during infection and transmission within the host tissue are associated with a variety of challenges, including exposure to physico-chemical factors that induce osmotic,...

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Published in:European journal of plant pathology 2020-02, Vol.156 (2), p.519-535
Main Authors: Przepiora, Tomasz, Figaj, Donata, Radzinska, Marta, Apanowicz (Bukrejewska), Malgorzata, Sieradzka, Malgorzata, Ambroziak, Patrycja, Hugouvieux-Cotte-Pattat, Nicole, Lojkowska, Ewa, Skorko-Glonek, Joanna
Format: Article
Language:English
Subjects:
Acidic oxides
Agriculture
Bacteria
Biomedical and Life Sciences
Dickeya solani
Disease transmission
DnaK protein
Ecology
Fitness
Genes
High temperature
Hydrogen peroxide
Life Sciences
Organic chemistry
Oxidants
Oxidative stress
Oxidizing agents
Plant Pathology
Plant Sciences
Potatoes
Structural stability
Thermal stress
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Summary:Dickeya solani is a pectinolytic bacterium that causes significant losses of potato crops. Interaction between bacteria and plant during infection and transmission within the host tissue are associated with a variety of challenges, including exposure to physico-chemical factors that induce osmotic, acidic, oxidative or thermal stresses. In this work, we tested the effects of various potentially adverse conditions on growth and fitness of the D. solani IPO2222 type strain. We found the bacteria were able to withstand a great variety of stressful conditions. Treatment with high concentrations of osmolytes (up to 1.6 Osm), low pH (5.0), or elevated temperature (up to 40 °C) was still tolerated. Short term exposure to these conditions did not impair the ability of bacteria to macerate potato tuber tissue. However, D. solani was sensitive to a relatively low content (above 0.5 mM) of the oxidant H 2 O 2 and treatment with this oxidant caused a loss of culturability. All used stressful conditions are known to affect the stability, and structure of proteins and induction of the folding stress-related genes, dnaK, dnaJ and groEL , was observed in most cases. The only exception was oxidative stress for which the chaperone genes were slightly down-regulated. This observation is in line with a low tolerance of D. solani to H 2 O 2 .
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-019-01902-z