Immunomodulatory properties of selectively processed prawn protein fractions assessed using human peripheral blood mononuclear cells

Summary Prawn muscles were treated with acetic acid and high‐pressure processing (600 MPa) separately to analyse their antigenicity and immunogenicity. The protein fractions were separated and isolated using preparative HPLC, and their antigenicity was analysed using Immunoglobulin G (IgG) ELISA kit...

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Bibliographic Details
Published in:International journal of food science & technology 2020-02, Vol.55 (2), p.795-804
Main Authors: Faisal, Md, Dargahi, Narges, Vasiljevic, Todor, Donkor, Osaana N.
Format: Article
Language:English
Subjects:
Acetic acid
Antigenicity
Antigens
Arginine
Arginine kinase
Cytokines
Enzyme-linked immunosorbent assay
Glucan
High-performance liquid chromatography
IgG antibody
Immunogenicity
Immunoglobulin E
Immunoglobulin G
Immunoglobulins
Immunomodulation
Inflammation
interleukin cytokine
Kinases
LC/MS/MS
Leukocytes (mononuclear)
Liquid chromatography
Methyltransferase
Muscles
Ovaries
PBMCs
Peripheral blood mononuclear cells
Phosphopyruvate hydratase
prawn allergy
Proteins
Tropomyosin
Tumors
Vitellogenin
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Summary:Summary Prawn muscles were treated with acetic acid and high‐pressure processing (600 MPa) separately to analyse their antigenicity and immunogenicity. The protein fractions were separated and isolated using preparative HPLC, and their antigenicity was analysed using Immunoglobulin G (IgG) ELISA kit. Out of thirty‐nine protein fractions, only four (A10, A11, B10 and C9) were detected with antigenic potentials. The immunogenicity of these protein fractions was analysed using human PBMCs, and supernatants were collected at multiple times from 0 to 144 h. The treated fractions (B10 and C9) analysed using Immunoglobulin E (IgE) ELISA kit showed significantly (P 
ISSN:0950-5423
1365-2621
DOI:10.1111/ijfs.14331