Construction of a red emission BODIPY-based probe for tracing lysosomal viscosity changes in culture cells

•A red emission lysosome-targeted viscosity probe was developed.•Fluorescence intensity of Lys-VBOD exhibit a good linear relationship with viscosity values.•Lys-VBOD can visualize the viscosity changes of lysosomes in live cells.•Photophysicochemical properties of Lys-VBOD is quite stable in physio...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2020-02, Vol.304, p.127271, Article 127271
Main Authors: Shen, Baoxing, Wang, Ling Feng, Zhi, Xu, Qian, Ying
Format: Article
Language:English
Subjects:
BODIPY
Dexamethasone
Emission spectra
Extreme values
Fluorescence
Living cell imaging
Lysosome labelling
Macromolecules
Quinoline
Red emission
Solvents
Titration
Twisting
Viscosity
Viscosity probe
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Summary:•A red emission lysosome-targeted viscosity probe was developed.•Fluorescence intensity of Lys-VBOD exhibit a good linear relationship with viscosity values.•Lys-VBOD can visualize the viscosity changes of lysosomes in live cells.•Photophysicochemical properties of Lys-VBOD is quite stable in physiological environment. A red emission BODIPY-based lysosome-targeted viscosity probe (Lys-VBOD) was synthesized for monitoring lysosomal viscosity in live cells. The increase of viscosity can restrict the twisting of double bond between indole and BODIPY fluorophore, also restrict the rotating of single bond between quinoline and BODIPY, which lead to the enhancement of fluorescence intensity in high viscosity environment. The changes of fluorescence intensity exhibit a good linear relationship with viscosity values. Besides, Lys-VBOD is quite stable in various pH solvents with given viscosity, suggesting that Lys-VBOD is suitable for applying in a wide pH range. Moreover, Lys-VBOD can visualize the changes of lysosomal viscosity in live cells under the stimulus of dexamethasone. BSA titration experiment demonstrated that Lys-VBOD is stable in the presence of macromolecules and proteins. Notably, the fluorescence lifetime of Lys-VBOD in large viscosity solvents is extremely high. These results indicate that Lys-VBOD is an excellent tool for detecing viscosity changes in vitro and live cells.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2019.127271