Gene note. Characterization of a tomato karyopherin [alpha] that interacts with the Tomato Yellow Leaf Curl Virus (TYLCV) capsid protein

A karyopherin [alpha] (LeKAP[alpha]1) cDNA was isolated from tomato plants. The deduced LeKAP[alpha]1 protein sequence of 527 amino acids showed similarity to other plant karyopherin [alpha] proteins. When LeKAP[alpha]1 was expressed in a yeast two-hybrid system together with the gene coding for the...

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Bibliographic Details
Published in:Journal of experimental botany 1999-05, Vol.50 (334), p.731
Main Authors: Kunik, T, Mizrachy, L, Citovsky, V, Gafni, Y
Format: Article
Language:English
Online Access:Get full text
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Summary:A karyopherin [alpha] (LeKAP[alpha]1) cDNA was isolated from tomato plants. The deduced LeKAP[alpha]1 protein sequence of 527 amino acids showed similarity to other plant karyopherin [alpha] proteins. When LeKAP[alpha]1 was expressed in a yeast two-hybrid system together with the gene coding for the capsid protein (CP) of the tomato yellow curl leaf virus (TYLCV), it interacted directly with CP. Thus, LeKAP[alpha]1 may be involved in the nuclear import of TYLCV CP and, potentially, the TYLCV genomes during viral infection of the host tomato cells. Keywords:Lycopersicon esculentum, karyopherin [alpha], TYLCV.
ISSN:0022-0957
1460-2431