Detection and Molecular Typing of Campylobacter Isolates from Human and Animal Faeces in Coastal Belt of Odisha, India

• Published in: Indian journal of medical microbiology 2019-07, Vol.37 (3), p.345-350
• Main Authors: Mohakud, Nirmal Kumar, Patra, Saumya Darshana, Kumar, Subrat, Sahu, Priyadarshi Soumyaranjan, Misra, Namrata, Shrivastava, Arpit Kumar
• Format: Article
• Language: English
• Subjects: Age; Animal species; Animals; Campylobacter; Campylobacter spp; Campylobacteriosis; Cattle; Concurrent infection; Deoxyribonucleic acid; Developing countries; Diarrhea; diarrhoea; DNA; DNA sequencing; Domestic animals; E coli; Epidemiology; Ethics; Feces; Food contamination; Gastroenteritis; Genetic testing; Goats; Infants; Infections; Infectious diseases; LDCs; Odisha; Pathogens; phylogenetic analysis; Phylogenetics; Polymerase chain reaction; Sheep; Signs and symptoms; Software; Studies; Water pollution; Water quality
• ISSN: 0255-0857; 1998-3646
• DOI: 10.4103/ijmm.IJMM_19_394

Introduction:Campylobacter-mediated diarrhoea is one of the major causes of gastroenteritis globally. A majority of the Campylobacter spp. that cause disease in humans have been isolated from animals. Faecal contamination of food and water is the identified frequent cause of human campylobacteriosis. Methodology: In the present study, faecal samples from patients with symptoms of acute diarrhoea (n = 310) and domestic animals including cows (n = 60), sheep (n = 45) and goats (n = 45) were collected from the same localities in the peri-urban Bhubaneswar city. Genomic DNA isolation followed by polymerase chain reaction and sequencing was employed to analyse Campylobacter spp.-positive samples. Results: Of the 460 faecal samples, 16.77% of human samples and 25.33% of animal samples were found to be positive for Campylobacter spp. Among animals, the isolation rate was highest in sheep followed by cows and goats with 9.33%, 8.66% and 7.33%, respectively. The highest number of Campylobacter-positive cases was diagnosed in infants of 2–5 years age. Concurrent infection of other pathogens in addition to Campylobacter spp. was frequently detected in the samples. Conclusion: The present study showed the incidence of Campylobacter infections in human and different animal species in and around Bhubaneswar, Odisha. The analysis suggested that domestic animals can be the potential sources for human campylobacteriosis in the region.