The regulation of the disease resistance, mTOR and NF-kB signaling pathway of Aristichthys nobilis using Rhodopseudomonas wastewater treatment

• Published in: Developmental and comparative immunology 2020-03, Vol.104, p.103517
• Main Authors: Wu, Pan, Liu, Yaxin, Li, Xiaoting, Gu, Yonghu, Liu, Yuxin, Hu, Yuqiao, Wang, Yanling, Wu, Yuan, Li, Ning, Zhang, Ying, Chen, Zhaobo, Jin, Hua
• Format: Article
• Language: English
• Subjects: Aquaculture; Aquaculture effluents; Aristichthys nobilis; Body composition; Disease resistance; Effluents; Fish diseases; Freshwater aquaculture; Gene expression; Medical treatment; Microorganisms; NF-κB protein; Phagocytes; Pollution control; Rhodopseudomonas; Signal transduction; Signaling; TOR protein; Wastewater discharges; Wastewater pollution; Wastewater treatment; Water discharge; Water pollution; Water quality; Water treatment
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2019.103517

The employment of traditional feed and medicament in freshwater aquaculture causes the frequent occurrence of environmental pollution and disease. Effluent collected after Rhodopseudomonas-mediated wastewater treatment could be re-utilized as microbial feeds, and aquaculture water to culture Aristichthys nobilis. Therefore, a novel integrated system of wastewater treatment using effluent containing Rhodopseudomonas that improves yield, increases disease resistance, and enhances the quality of aquaculture water for Aristichthys nobilis culture was proposed and investigated. Aristichthys nobilis can grow well in effluent containing Rhodopseudomonas (ER). The survival rate, yield, and whole body composition of the ER group were all increased compared to the control group (CK). The biochemical (B vitamin) and other substances in the effluent of Rhodopseudomonas enhanced the activity of AKP, ACP, phagocytic, SOD, and CAT by upregulating the expression of AKP, ACP, SOD, and CAT genes. Moreover, Rhodopseudomonas and biochemical substances improved mTOR and NF-kB signaling pathway. Furthermore, Rhodopseudomonas inhibited Aeromonas hydrophila that increases resistance against fish disease. Meanwhile, Rhodopseudomonas in the effluent also improved the aquaculture water quality. This technology would save the aquaculture water, reduce water pollution and wastewater discharge, and increase the output and disease resistance of Aristichthys nobilis, simultaneously.