Experimental design for the production of tensio-active agent by Candida lipolytica

The strategy of optimization using sequential factorial design was employed to enhance the tensio-active emulsifying agent produced by Candida lipolytica using soybean oil refinery residue as substrate. A full factorial design was used to evaluate the impact of three fermentation factors--amounts of...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2008-08, Vol.35 (8), p.907-914
Main Authors: Rufino, Raquel D, Sarubbo, Leonie A, Neto, Benício B, Campos-Takaki, Galba M
Format: Article
Language:English
Subjects:
Acids
Biochemistry
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Candida - metabolism
Culture Media - chemistry
Design
Emulsifying Agents - metabolism
Experimental design
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Glutamic Acid - metabolism
Hydrogen-Ion Concentration
Industrial Waste
Industrial wastes
Inorganic Chemistry
Life Sciences
Microbiology
Microorganisms
Optimization
Original Paper
Peptones - metabolism
Sodium Chloride
Soybean oil
Soybean Oil - metabolism
Soybeans
Studies
Surface Tension
Surfactants
Temperature
Time Factors
Variables
Vegetable oils
Yeast
Yeasts
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Summary:The strategy of optimization using sequential factorial design was employed to enhance the tensio-active emulsifying agent produced by Candida lipolytica using soybean oil refinery residue as substrate. A full factorial design was used to evaluate the impact of three fermentation factors--amounts of refinery residue, glutamic acid and yeast extract. This allowed exclusion of the yeast extract. Full factorials designs were then sequentially used to optimize the levels of the residue and glutamic acid. The surface tension value was finally reduced to 25.29 mN/m. The maximum emulsifier activity using different substrates was within 40 h of cultivation. The surface tension of the cell-free broth containing the biosurfactant remained very stable during exposure to a wide range of pH (2-12), temperatures (0-120°C) and salinity (2-10% NaCl). The combination of an industrial waste and a cheap substrate therefore seems to be very promising for the low-cost production of potent biosurfactant.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-008-0364-3