Visfatin Exerts Immunotherapeutic Effects in Lipopolysaccharide-Induced Acute Lung Injury in Murine Model

• Published in: Inflammation 2020-02, Vol.43 (1), p.109-122
• Main Authors: Luo, You, Pang, Xin-Xin, Ansari, Abdur Rahman, Wu, Xin-Tong, Li, Hui-Zhen, Zhang, Zhe-Wei, Song, Hui
• Format: Article
• Language: English
• Subjects: Acute Lung Injury - chemically induced; Acute Lung Injury - immunology; Acute Lung Injury - metabolism; Acute Lung Injury - prevention & control; Animal models; Animals; Anti-Inflammatory Agents - pharmacology; Antioxidants; Biomedical and Life Sciences; Biomedicine; Cell number; Cytokines; Cytokines - metabolism; Cytokines - pharmacology; Disease Models, Animal; IL-1β; Immune response; Immunology; Inflammation; Inflammation Mediators - metabolism; Interleukin 10; Interleukin 6; Internal Medicine; Lipopolysaccharides; Lung - drug effects; Lung - immunology; Lung - metabolism; Lung - pathology; Lungs; Lymphocytes; Mice; mRNA; Neutrophils; Nicotinamide Phosphoribosyltransferase - pharmacology; Original Article; Pathology; Peripheral blood; Peroxidase; Peroxidase - metabolism; Pharmacology/Toxicology; Polymerase chain reaction; Pulmonary Fibrosis - chemically induced; Pulmonary Fibrosis - immunology; Pulmonary Fibrosis - metabolism; Pulmonary Fibrosis - prevention & control; Rheumatology; TLR4 protein; Toll-like receptors; Transforming Growth Factor beta1 - metabolism; Tumor necrosis factor-α; Western blotting; γ-Interferon
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-019-01100-3

Visfatin acts as a significant regulator of inflammatory cytokines. However, the immunological response and therapeutic effects of visfatin under bacterial stress in murine lung tissue are still not clear. To investigate the role of visfatin on lipopolysaccharide (LPS)-induced acute lung injury (ALI), thirty Kunming mice were divided into Saline, LPS, and LPS + visfatin groups. After routine blood examination, the effects of visfatin on inflammatory cytokines, lung tissue structure, and expression of inflammatory mediators were explored through hematoxylin-eosin (H&E), Masson and immunohistochemical staining, quantitative polymerase chain reaction (Q-PCR), and Western blotting. Compared with the Saline group, neutrophil percentage, peripheral blood neutrophil count, and the ratio of lymphocyte count (NLR) were upregulated in LPS group. Moreover, Masson staining showed alterations in lung tissue structure; the mRNA level of different cytokines (IL-6, IL-1β, TNF-α, IL-10, TLR4, IFN-γ) was upregulated; and the protein expression of interleukin (IL)-6, myeloperoxidase (MPO), and transforming growth factor-β1 (TGF-β) was significantly ( p