Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection

• Published in: Euphytica 2010-05, Vol.173 (2), p.279-287
• Main Authors: de Oliveira, Eder Jorge, dos Santos Silva, Aline, de Carvalho, Fabiana Moraes, dos Santos, Lucas Ferraz, Costa, Juliana Leles, de Oliveira Amorim, Vanusia Batista, Dantas, Jorge Luiz Loyola
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; alleles; artificial selection; Biological and medical sciences; Biomarkers; Biomedical and Life Sciences; Biotechnology; Carica papaya; Citrus fruits; Classical and quantitative genetics. Population genetics. Molecular genetics; Fundamental and applied biological sciences. Psychology; gene segregation; Generalities. Genetics. Plant material; Genetic diversity; genetic markers; genetic polymorphism; genetic variation; Genetics and breeding of economic plants; homozygosity; Inbreeding; inbreeding coefficient; Life Sciences; marker-assisted selection; microsatellite repeats; Molecular biology; Molecular genetics; molecular sequence data; papayas; plant breeding; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Polymorphism; selection criteria; Varietal selection. Specialized plant breeding, plant breeding aims
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-010-0150-y

Conventional methods for the selection of papaya pure lines are time-consuming. Procedures involving the use of molecular markers and the indirect selection of homozygous plants can reduce this time considerably. The objective of this study was to evaluate the informativeness of a microsatellite marker set when used in marker-assisted selection (MAS) for the development of new papaya lines. Eighty-three lines originating from two segregating F₃ populations and from papaya germplasm were used for the molecular analysis of 27 microsatellite primers. Twenty polymorphic microsatellite primers were identified, allowing the identification of 86 alleles, with an average of 3.18 alleles per primer. The observed heterozygosity values were low for both the markers (0.00-0.29) and the individual lines (0.00-0.35). The inbreeding coefficient (f) ranged from 0.634 to 1.00. Eleven lines with f = 1.00 and 18 lines with f varying from 0.953 to 0.961 were identified. In addition, papaya lines showed high genetic diversity, which will certainly contribute to the development of new varieties. Our results show that the use of microsatellites in MAS is a quick and effective procedure for the development of papaya lines.