Sensitive and selective detection of microRNA in complex biological samples based on protein-enhanced fluorescence anisotropy

Here we report a molecular mass amplifying strategy to construct a fluorescence anisotropy sensor for the detection of microRNA. Because fluorescence anisotropy is less affected by biological matrix interferences, the proposed method can be used directly for the detection of targets in tumor cell ly...

Full description

Saved in:
Bibliographic Details
Published in:Analytical methods 2020-02, Vol.12 (5), p.687-692
Main Authors: Li, Yan, Sun, Yao, Ye, Jieyun, Pan, Fengping, Peng, Bo, Li, Haiqiang, Zhang, Mingming, Xu, Yan
Format: Article
Language:English
Subjects:
Anisotropy
Biological properties
Biological samples
Fluorescence
Linearity
Lysates
MicroRNAs
miRNA
Pretreatment
Ribonucleic acid
RNA
Target detection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Here we report a molecular mass amplifying strategy to construct a fluorescence anisotropy sensor for the detection of microRNA. Because fluorescence anisotropy is less affected by biological matrix interferences, the proposed method can be used directly for the detection of targets in tumor cell lysates without any tedious sample pretreatment, and a good linearity in the 10 pM to 0.5 nM range with a detection limit down to 3.4 pM was achieved. The results established that our method can be used to design fluorescence anisotropy sensors for rapid, sensitive, and selective detection of miRNAs for early diagnosis of cancer. A molecular mass amplifying strategy to construct a fluorescence anisotropy sensor for the detection of microRNA with high sensitivity and selectivity in complex biological samples was proposed.
ISSN:1759-9660
1759-9679
DOI:10.1039/c9ay02024a