Comparison of 2D and 3D cell cultures of colorectal adenocarcinoma as models for drug screening

The cytostatic effects of 5-fluorouracil, oxaliplatin, and 4-(5-methyl-5 H -[1,3]dioxolo[4,5- f ]-indol-7-yl)-3-(5-fluoro-1-benzofuran-3-yl)-1 H -pyrrole-2,5-dione (9-ING-41), a new glycogen synthase kinase-3 inhibitor, were evaluated in 2D and 3D models of HT-29 colorectal adenocarcinoma cells. The...

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Bibliographic Details
Published in:Russian chemical bulletin 2019-12, Vol.68 (12), p.2377-2380
Main Authors: Poloznikov, A. A., Nikulin, S. V., Bolotina, L. V., Gaisina, I. N., Alexeev, B. Ya
Format: Article
Language:English
Subjects:
Brief Communications
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Cytotoxicity
Glycogens
Inorganic Chemistry
Kinases
Organic Chemistry
Three dimensional models
Two dimensional models
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Summary:The cytostatic effects of 5-fluorouracil, oxaliplatin, and 4-(5-methyl-5 H -[1,3]dioxolo[4,5- f ]-indol-7-yl)-3-(5-fluoro-1-benzofuran-3-yl)-1 H -pyrrole-2,5-dione (9-ING-41), a new glycogen synthase kinase-3 inhibitor, were evaluated in 2D and 3D models of HT-29 colorectal adenocarcinoma cells. The HT-29 cells cultured in 3D were resistant to the test drugs, while in the case of the 2D model, not only cytostatic but also cytotoxic effect was observed. The highest cytotoxic effect was detected upon the addition of 9-ING-41, at high concentrations of which the cell viability decreased to 50% after 3 h of incubation. The results indicate that 3D cultures reproduce the tumor structure and drug resistance more accurately.
ISSN:1066-5285
1573-9171
DOI:10.1007/s11172-019-2716-8