Coexpression of Vimentin and Cytokeratins in Human Melanoma Cell Lines

—Molecular heterogeneity in seven human metastatic melanoma cells lines is revealed using a double immunofluorescent assay by flow cytometry. It is evaluated that quite a small population of cells (up to 10%) expressed only vimentin, the main marker of melanoma. Only epithelial cytokeratins expressi...

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Bibliographic Details
Published in:Moscow University chemistry bulletin 2019-11, Vol.74 (6), p.296-299
Main Authors: Bogush, T. A., Kaliuzhny, S. A., Basharina, A. A., Bogush, E. A., Kirsanov, V. Yu, Kosorukov, V. S., Burova, O. S., Davydov, M. M., Baryshnikova, M. A.
Format: Article
Language:English
Subjects:
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Flow cytometry
Markers
Melanoma
Metastasis
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Summary:—Molecular heterogeneity in seven human metastatic melanoma cells lines is revealed using a double immunofluorescent assay by flow cytometry. It is evaluated that quite a small population of cells (up to 10%) expressed only vimentin, the main marker of melanoma. Only epithelial cytokeratins expression is revealed in a significantly greater number of cells (from 25 to 50%). The dominant molecular phenotype (50–80% cells expressed both markers), the coexpression of vimentin and cytokeratins, is identified for the first time. The authors assume that the latter phenotype of melanoma cells may be a molecular marker of the tumor’s aggressiveness and metastatic potential.
ISSN:0027-1314
1935-0260
DOI:10.3103/S0027131419060075