Effect of amino acids and B-group vitamins on nucleation of calcium oxalate monohydrate crystals

• Published in: Journal of crystal growth 2020-02, Vol.531, p.125368, Article 125368
• Main Authors: Taranets, Y.V., Bezkrovnaya, O.N., Pritula, I.M.
• Format: Article
• Language: English
• Subjects: A1. Biocrystallization; A1. Nucleation; A1. Supersaturated solutions; A2. Growth from solutions; Amino acids; B1. Calcium compounds; B1. Nanomaterials; Calcium; Crystal growth; Crystallization; Crystals; Kidney stones; Nucleation; Surface energy; Vitamins
• ISSN: 0022-0248; 1873-5002
• DOI: 10.1016/j.jcrysgro.2019.125368

[Display omitted] •L-Thr amino acid promotes the processes of COM nucleation.•B1 and B6 vitamins inhibit the COM growth; the induction time increases in 70 times.•The degree of inhibition of COM crystal growth with 50 mM B1 or B6 exceeded 95%.•Additive the L-Asp, L-Arg, B1 and B6 reduce the COM surface energy. Studied is the influence of amino acids (L-Asp, L-Arg, L-Thr) and B-group vitamins (B1, B6, B12) on the processes of nucleation and the value of surface energy of calcium oxalate monohydrate (COM) crystals. It is established that L-Asp and L-Arg molecules inhibit nucleation of COM, that increases their induction time tenfold in comparison with the one of the pure crystals. L-Thr amino acid is shown to promote the processes of COM nucleation and to diminish the induction time by two times. At the introduction of L-Asp and L-Arg molecules into the solution the degree of COM crystal growth inhibition is 32.0–90.2% and 10.2–86.0% respectively. It is found that B1 and B6 vitamins inhibit the growth of COM crystals practically completely (the inhibition degree exceeds 95%) and lead to 70-fold increase in the induction time of pure COM. The value of surface energy of COM crystals diminishes to 19.8–20.8 mJ/m2 at the introduction of the crystallization inhibitor molecules (L-Asp, L-Arg, B1, B6) into COM solution.