Piceatannol Inhibits Akt Activation, Induces G2/M Phase Arrest and Mitochondrial Apoptosis and Augments Cisplatin Efficacy in U2OS Osteosarcoma Cells

ABSTRACT Piceatannol (3,3',4,5'-tetrahydroxy-trans-stilbene), a naturally occurring analog of resveratrol has been shown to exhibit anticancer activity in various human cancer cells. However, the anticancer mechanism of piceatannol is not well studied. In the meantime, cisplatin (CDDP) is...

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Bibliographic Details
Published in:Pakistan journal of zoology 2019-12, Vol.51 (6), p.2337
Main Authors: Li, Zijuan, Ma, Rong, Khan, Muhammad, Liu, Chenchen, Cui, Xiaolin, Li, Yongming
Format: Article
Language:English
Subjects:
AKT protein
Anticancer properties
Antitumor activity
Apoptosis
Bcl-2 protein
Biomedical materials
Bone cancer
Cancer
Cancer therapies
Cancer treatment
Care and treatment
Cell culture
Cell cycle
Cell growth
Cell proliferation
Chemotherapy
Cholecystokinin
Cisplatin
Drug dosages
Drug resistance
Flow cytometry
Immunoglobulins
Medical research
Mitochondria
Morphology
Novels
Osteosarcoma
Osteosarcoma cells
Piceatannol
Proteins
Resveratrol
Stilbene
Surgery
trans-Stilbene
Tumors
Youth
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Summary:ABSTRACT Piceatannol (3,3',4,5'-tetrahydroxy-trans-stilbene), a naturally occurring analog of resveratrol has been shown to exhibit anticancer activity in various human cancer cells. However, the anticancer mechanism of piceatannol is not well studied. In the meantime, cisplatin (CDDP) is the first line treatment for some tumors. The present study was aimed to explore the cellular targets and anticancer mechanism of piceatannol in human osteosarcoma U2OS cells. Cell proliferation was measured using CCK-8 assay and colony forming assay. Flow cytometry was used to determine apoptosis and cell cycle profile. Western blot was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase arrest and apoptosis. Further studies showed that piceatannol induces mitochondrial apoptosis as shown by Bcl-2 family proteins modulation. Finally, piceatannol significantly enhanced apoptotic efficacy of CDDP in U2OS cells. On the basis of our findings, piceatannol is a promising anticancer agent which could be developed into lead for osteosarcoma treatment as a single agent or in combination with CDDP.
ISSN:0030-9923
DOI:10.17582/journal.pjz/2019.51.6.2337.2344