Effects of di (2-ethylhexyl) phthalate and high-fat diet on lipid metabolism in rats by JAK2/STAT5

• Published in: Environmental science and pollution research international 2020-02, Vol.27 (4), p.3837-3848
• Main Authors: Zhang, Yuezhu, Zhou, Liting, Zhang, Zhaoming, Xu, Qi, Han, Xu, Zhao, Yaming, Song, Xinyue, Zhao, Tianyang, Ye, Lin
• Format: Article
• Language: English
• Subjects: Accumulation; Adipogenesis; Animals; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Diet; Diet, High-Fat; Diethylhexyl Phthalate; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Exposure; Fat metabolism; High fat diet; Janus kinase 2; Janus Kinase 2 - chemistry; Janus Kinase 2 - metabolism; Lipid Metabolism; Lipids; Liver; Metabolic disorders; Morphology; mRNA; Phthalates; Phthalic Acids - chemistry; Rats; Rats, Wistar; Research Article; Stat5 protein; STAT5 Transcription Factor - chemistry; STAT5 Transcription Factor - metabolism; Toxicity; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-019-06599-5

Exposure to di (2-ethylhexyl) phthalate (DEHP) induces lipid metabolism disorder and high-fat diet (HD) may have joint effects with DEHP. We aim to clarify the role of JAK2/STAT5 pathway in the process and reveal the effects of HD on the toxicity of DEHP. Wistar rats (160 animals) were fed with HD or normal diet (ND) respectively and exposed to DEHP 0, 5, 50, and 500 mg/kg/day for 8 weeks. Lipid levels, as well as the morphology of liver and adipose, mRNA levels, and protein levels of JAK2, STAT5A, STAT5B, FAS, ap2, and PDK4 were detected. The results showed that DEHP exposure leads to increased weight gain. The JAK2/STAT5 pathway was activated in adipose after DEHP exposure and promoted the expression of FAS, ap2, and PDK4 in ND rats. While in the liver, JAK2 was inhibited, and lipid synthesis and accumulation were increased. However, rats exposed to DEHP in combination with HD showed a complete disorder of lipid metabolism. Therefore, we conclude that DEHP affects lipid metabolism through regulating the JAK2/STAT5 pathway and promotes adipogenesis and lipid accumulation. High-fat diet may have a joint effect with DEHP on lipid metabolism disorder.