Mirror‐Image 5S Ribonucleoprotein Complexes

After realizing mirror‐image genetic replication, transcription, and reverse transcription, the biggest challenge in establishing a mirror‐image version of the central dogma is to build a mirror‐image ribosome‐based translation machine. Here, we chemically synthesized the natural and mirror‐image ve...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie 2020-02, Vol.132 (9), p.3753-3760
Main Authors: Ling, Jun‐Jie, Fan, Chuyao, Qin, Hong, Wang, Min, Chen, Ji, Wittung‐Stafshede, Pernilla, Zhu, Ting F.
Format: Article
Language:English
Subjects:
Chemische Proteinsynthese
Chemistry
Chiralität
E coli
Festphasenpeptidsynthese
Native chemische Ligation
Organic chemistry
Protein interaction
Proteins
Reverse transcription
Ribonucleic acid
Ribonucleoproteine
Ribosomal proteins
RNA
rRNA 5S
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:After realizing mirror‐image genetic replication, transcription, and reverse transcription, the biggest challenge in establishing a mirror‐image version of the central dogma is to build a mirror‐image ribosome‐based translation machine. Here, we chemically synthesized the natural and mirror‐image versions of three ribosomal proteins (L5, L18, and L25) in the large subunit of the Escherichia coli ribosome with post‐translational modifications. We show that the synthetic mirror‐image proteins can fold in vitro despite limited efficiency and assemble with enzymatically transcribed mirror‐image 5S ribosomal RNA into ribonucleoprotein complexes. In addition, the RNA–protein interactions are chiral‐specific in that the mirror‐image ribosomal proteins do not bind with natural 5S ribosomal RNA and vice versa. The synthesis and assembly of mirror‐image 5S ribonucleoprotein complexes are important steps towards building a functional mirror‐image ribosome. Die natürlichen und spiegelbildlichen Varianten von drei ribosomalen Proteinen (L5, L18 und L25) wurden mit posttranslationalen Modifikationen chemisch synthetisiert. Die synthetischen spiegelbildlichen Proteine können sich trotz begrenzter Effizienz in vitro falten und mit enzymatisch transkribierter, spiegelbildlicher 5S‐ribosomaler RNA Ribonucleoprotein‐Komplexe bilden.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201914799