RNAi for management of Asian long-horned beetle, Anoplophora glabripennis: identification of target genes

The Asian long-horned beetle (ALB), Anoplophora glabripennis, is a serious invasive forest pest in several countries, including the USA. Currently, there are no effective methods available to manage this pest without adverse impacts on the environment, but RNA interference (RNAi) technology may prov...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pest science 2020-03, Vol.93 (2), p.823-832
Main Authors: Dhandapani, Ramesh Kumar, Gurusamy, Dhandapani, Duan, Jian Jun, Palli, Subba Reddy
Format: Article
Language:English
Subjects:
Adults
Agriculture
Anoplophora glabripennis
Apoptosis
Bacteria
Biomedical and Life Sciences
Double-stranded RNA
Ecology
Entomology
Environmental impact
Forestry
Gene expression
Genes
Invasive insects
Larvae
Life Sciences
Microinjection
Mortality
Oral administration
Original Paper
Pest control
Pests
Plant Pathology
Plant Sciences
Protein transport
Ribonucleic acid
RNA
RNA-mediated interference
Target recognition
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Asian long-horned beetle (ALB), Anoplophora glabripennis, is a serious invasive forest pest in several countries, including the USA. Currently, there are no effective methods available to manage this pest without adverse impacts on the environment, but RNA interference (RNAi) technology may provide a more environmentally friendly pest control option. To identify target genes for control of ALB, we screened 48 candidate genes in larvae and selected several effective target genes. Injection of double-stranded RNA (dsRNA) targeting the gene coding for the inhibitor of apoptosis (dsIAP) or vacuolar sorting protein, SNF7 (dsSNF7), into larvae and adults resulted in a significant knockdown of these genes and caused 100% mortality in both larvae and adults. As microinjection of dsRNA is not a feasible option for field applications, delivery through oral route was then attempted. Oral delivery of naked dsRNA did not cause mortality in larvae and adults. However, feeding heat-killed bacteria containing dsIAP or dsSNF7 induced mortality and knockdown of target genes in larvae and adults. In conclusion, the utilization of heat-killed bacteria for delivery of dsRNA targeting IAP or SNF7 gene may serve as a potential pest control method for ALB.
ISSN:1612-4758
1612-4766
DOI:10.1007/s10340-020-01197-8