Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Anthocyanins (AC) from Coreopsis tinctoria possesses strong antioxidant properties, while the effects of AC on cells damage induced by reactive oxygen species (ROS) in diabetes mellitus diseases progression have not been reported. The present study was carried out to evaluate the protective property...

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Bibliographic Details
Published in:Chemistry & biodiversity 2020-02, Vol.17 (2), p.e1900587-n/a
Main Authors: Liu, Jianli, Tian, Siqi, Fu, Mingyang, He, Yin, Yu, Hui, Cao, Xiangyu, Cao, Yiyang, Xu, Hanyuan
Format: Article
Language:English
Subjects:
Animals
Anthocyanins
Anthocyanins - chemistry
Anthocyanins - pharmacology
antioxidant capacity
Antioxidants
Apoptosis
Apoptosis - drug effects
Catalase - metabolism
Cell Line
Cell Nucleus - metabolism
Cell Survival - drug effects
Cell viability
Cellular manufacture
Coreopsis - chemistry
Coreopsis - metabolism
Coreopsis tinctoria
Diabetes mellitus
Hydrogen peroxide
Hydrogen Peroxide - toxicity
Injury analysis
Mice
NF-E2-Related Factor 2 - metabolism
Oxidation
Oxidative stress
Oxidative Stress - drug effects
p38 MAPK
Phosphorylation
Protective Agents - chemistry
Protective Agents - pharmacology
Reactive oxygen species
Reactive Oxygen Species - metabolism
Superoxide dismutase
Superoxide Dismutase - metabolism
Translocation
Up-Regulation - drug effects
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Summary:Anthocyanins (AC) from Coreopsis tinctoria possesses strong antioxidant properties, while the effects of AC on cells damage induced by reactive oxygen species (ROS) in diabetes mellitus diseases progression have not been reported. The present study was carried out to evaluate the protective property of AC against cellular oxidative stress with an experimental model, H2O2‐exposed MIN6 cells. AC could reverse the decrease of cell viability induced by H2O2 and efficiently suppressed cellular ROS production and cell apoptosis. In addition, Real‐time PCR and Western blot analyses indicated that AC could protect MIN6 cells against oxidative injury through increasing the translocation of Nrf2 into nuclear, decreasing the phosphorylation level of p38 and up‐regulating the protein expression of antioxidant enzyme (SOD1, SOD2 and CAT). Thus, this study provides evidence to support the beneficial effect of AC in inhibiting MIN6 cells from H2O2‐induced oxidative injury.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.201900587