Effect of external oxygen demand on radial oxygen loss by Juncus roots in titanium citrate solutions

• Published in: Plant, cell and environment cell and environment, 1999-12, Vol.22 (12), p.1587-1593
• Main Author: Sorrell, B. K.
• Format: Article
• Language: English
• Subjects: aeration; Biological and medical sciences; diffusion; Fundamental and applied biological sciences. Psychology; Juncus effusus; Juncus inflexus; Metabolism; oxygen; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; roots
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1046/j.1365-3040.1999.00517.x

ABSTRACT Radial oxygen loss (ROL) from the roots of two semiaquatic rushes, Juncus effusus L. and Juncus inflexus L., was studied in reducing titanium citrate buffer, using both closed incubations and a flow‐through, titrimetric system. In closed experiments, roots released oxygen at a constant rate over a wide range of external oxygen demands, with the ROL rate only depending on sink strength at low demands, and no oxygen release into oxidized solutions. In the titrimetric experiments, roots continued to release oxygen at constant rates when provided with a constant external oxygen demand. ROL was higher in J. effusus (9·5 ± 1 × 10−7 mol O2 h−1 root−1) than in J. inflexus (4·5 ± 0·5 × 10−7 mol O2 h−1 root−1). Light and dark changes around the shoots did not affect the ROL rate in J. inflexus, whereas in J. effusus ROL was ≈ 1·75 times higher in the light than in the dark, presumably due to changes in stomatal aperture. These results suggest that ROL is controlled by the external oxygen demand at low to moderate reducing intensities, but that structural limitations to oxygen diffusion rates prevent ROL from continuing to increase at higher external oxygen demands.