Death-associated protein 5 (DAP5/p97/NAT1) contributes to retinoic acid-induced granulocytic differentiation and arsenic trioxide-induced apoptosis in acute promyelocytic leukemia

All-trans-retinoic acid (ATRA) and arsenic trioxide (ATO) induce differentiation and apoptosis in acute promyelocytic leukemia (APL) cells. Here we investigated the role and regulation of death-associated protein-5 (DAP5/p97/NAT1), a novel inhibitor of translational initiation, in APL cell different...

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Bibliographic Details
Published in:Apoptosis (London) 2008-07, Vol.13 (7), p.915-928
Main Authors: Ozpolat, Bulent, Akar, Ugur, Zorrilla-Calancha, Isabel, Vivas-Mejia, Pablo, Acevedo-Alvarez, Marian, Lopez-Berestein, Gabriel
Format: Article
Language:English
Subjects:
Apoptosis - drug effects
Apoptosis - physiology
Apoptosis Regulatory Proteins - metabolism
Arsenic
Arsenic Trioxide
Arsenicals - pharmacology
Base Sequence
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cell Biology
Cell differentiation
Cell Differentiation - drug effects
Cell Line, Tumor
Eukaryotic Initiation Factor-2 - metabolism
Eukaryotic Initiation Factor-4G - antagonists & inhibitors
Eukaryotic Initiation Factor-4G - genetics
Eukaryotic Initiation Factor-4G - metabolism
Granulocytes - drug effects
Granulocytes - metabolism
Granulocytes - pathology
HL-60 Cells
Humans
Leukemia
Leukemia, Promyelocytic, Acute - drug therapy
Leukemia, Promyelocytic, Acute - metabolism
Leukemia, Promyelocytic, Acute - pathology
Models, Biological
Oncology
Original Paper
Oxides - pharmacology
Phosphatidylinositol 3-Kinases - metabolism
Protein Kinases - metabolism
Proto-Oncogene Proteins c-akt - metabolism
RNA, Small Interfering - genetics
RNA-Binding Proteins - metabolism
Signal Transduction
TOR Serine-Threonine Kinases
Translocation
Tretinoin - pharmacology
Up-Regulation - drug effects
Virology
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Summary:All-trans-retinoic acid (ATRA) and arsenic trioxide (ATO) induce differentiation and apoptosis in acute promyelocytic leukemia (APL) cells. Here we investigated the role and regulation of death-associated protein-5 (DAP5/p97/NAT1), a novel inhibitor of translational initiation, in APL cell differentiation and apoptosis. We found that ATRA markedly induced DAP5/p97 protein and gene expression and nuclear translocation during terminal differentiation of APL (NB4) and HL60 cells but not differentiation-resistant cells (NB4.R1 and HL60R), which express very low levels of DAP5/p97. At the differentiation inducing concentrations, ATO (
ISSN:1360-8185
1573-675X
DOI:10.1007/s10495-008-0222-9