Characteristics of Plasma Activated Medium Produced by Atmospheric Pressure Helium Plasma Jet and Its Selective Effect on Malignant Melanoma and Normal Fibroblast Cells

The characteristics of plasma activated medium (PAM) and its different effects on cancer and normal cells are a relevant topic of study in the field of plasma medicine. In this study, an atmospheric pressure plasma jet (APPJ) with helium was applied for generating PAM, which was utilized for maligna...

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Bibliographic Details
Published in:IEEE transactions on plasma science 2020-02, Vol.48 (2), p.587-595
Main Authors: Xu, Gui-Min, Hao, Yue, Sun, Ming-Yang, Liu, Jin-Ren, Shi, Xing-Min, Zhang, Guan-Jun
Format: Article
Language:English
Subjects:
Apoptosis
Atmospheric pressure
Atmospheric pressure plasma jet (APPJ)
Cells (biology)
Electrodes
fibroblast
Fibroblasts
Flow rates
Flow velocity
Gas flow
Helium
Helium plasma
Hydrogen peroxide
Melanoma
melanoma cells
plasma activated medium (PAM)
Plasma jets
selective effect
Skin cancer
Vapor phases
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Summary:The characteristics of plasma activated medium (PAM) and its different effects on cancer and normal cells are a relevant topic of study in the field of plasma medicine. In this study, an atmospheric pressure plasma jet (APPJ) with helium was applied for generating PAM, which was utilized for malignant melanoma (B16) and normal fibroblast (3T3) cell treatment. With the applied peak-peak voltages of 8, 10, and 12 kV, together with the gas flow rates of 1, 2, and 3 L/min, the characteristics of He APPJ and PAM were investigated, respectively. The results showed that compared with the free-standing jet, both the total power that was dissipated into the APPJ device and the emission intensities of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the gas phase increased when it interacted with the medium. There were obvious increases of H 2 O 2 and NO 2 - in PAM with different APPJ activation time of 5, 10, 15, 30, 45, and 60 s. The gas flow rate did not yield significant differences in the concentration of species generated, the applied voltage being a more relevant parameter. Finally, the results of cell viability and cell apoptosis assay indicated that the identical dose PAM had a selective effect on two kinds of nonhomologous cells.
ISSN:0093-3813
1939-9375
DOI:10.1109/TPS.2020.2965564