Colloidally stable polypeptide‐based nanogel: Study of enzyme‐mediated nanogelation in inverse miniemulsion

ABSTRACT The current work presents a pivotal study of the nanogelation of the linear poly(N5‐2‐hydroxypropyl‐L‐glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2‐mediated crosslinking. The effects of various nH2O2/nTYR ratios on the...

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Published in:Journal of applied polymer science 2020-06, Vol.137 (21), p.n/a
Main Authors: Dvořáková, Jana, Šálek, Petr, Korecká, Lucie, Pavlova, Ewa, Černoch, Peter, Janoušková, Olga, Koutníková, Barbora, Proks, Vladimír
Format: Article
Language:English
Subjects:
biodegradable
Biodegradation
biomedical applications
Blood plasma
colloids
Crosslinking
Cryoforming
Fibroblasts
Fluorescence
Glutamine
Hydrogen peroxide
Materials science
Nanoparticles
Peroxidase
Photon correlation spectroscopy
Polymers
Polypeptides
Prepolymers
Reaction kinetics
Reaction time
synthesis and processing techniques
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Summary:ABSTRACT The current work presents a pivotal study of the nanogelation of the linear poly(N5‐2‐hydroxypropyl‐L‐glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2‐mediated crosslinking. The effects of various nH2O2/nTYR ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio nH2O2/nTYR = 2, the strategy yields in the high‐quality ~ 130 nm poly(amino acid)‐based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48725.
ISSN:0021-8995
1097-4628
DOI:10.1002/app.48725