Simultaneous Quantitative Determination of Amphetamines, Opiates, Ketamine, Cocaine and Metabolites in Human Hair: Application to Forensic Cases of Drug Abuse

A method using liquid chromatography–tandem mass spectrometry (LC–MS/MS) to simultaneously quantify amphetamines, opiates, ketamine, cocaine, and metabolites in human hair is described. Hair samples (50 mg) were extracted with methanol utilizing cryogenic grinding. Calibration curves for all the ana...

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Bibliographic Details
Published in:Journal of forensic sciences 2020-03, Vol.65 (2), p.563-569
Main Authors: Zhuo, Yue, Wang, Xin, Wu, Jingjie, Zhang, Sujing, Deng, Hongxiao, Liu, Wei, Wu, Hejian, Xiang, Ping, Shen, Min
Format: Article
Language:English
Subjects:
Adult
Amphetamines
Amphetamines - analysis
Chromatography, Liquid
Cocaine
Cocaine - analysis
Drug abuse
Drugs
Female
forensic science
forensic toxicology
Forensic Toxicology - methods
Hair
Hair - chemistry
hair analysis
Humans
Ketamine
Ketamine - analysis
Limit of Detection
Liquid chromatography
liquid chromatography–tandem mass spectrometry
Male
Mass spectrometry
Metabolites
metabolite‐to‐drug concentration ratio
Middle Aged
Narcotics
Narcotics - analysis
Opiate Alkaloids - analysis
opiates
Substance Abuse Detection - methods
Substance-Related Disorders - diagnosis
Tandem Mass Spectrometry
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Summary:A method using liquid chromatography–tandem mass spectrometry (LC–MS/MS) to simultaneously quantify amphetamines, opiates, ketamine, cocaine, and metabolites in human hair is described. Hair samples (50 mg) were extracted with methanol utilizing cryogenic grinding. Calibration curves for all the analytes were established in the concentration range 0.05–10 ng/mg. The recoveries were above 72%, except for AMP at the limit of quantification (LOQ), which was 48%. The accuracies were within ±20% at the LOQ (0.05 ng/mg) and between −11% and 13.3% at 0.3 and 9.5 ng/mg, respectively. The intraday and interday precisions were within 19.6% and 19.8%, respectively. A proficiency test was applied to the validated method with z‐scores within ±2, demonstrating the accuracy of the method for the determination of drugs of abuse in the hair of individuals suspected of abusing drugs. The hair concentration ranges, means, and medians are summarized for abused drugs in 158 authentic cases.
ISSN:0022-1198
1556-4029
DOI:10.1111/1556-4029.14179