FDS, a novel saponin isolated from Felicium decipiens: Lectin interaction and biological complementary activities

• Published in: Process biochemistry (1991) 2020-01, Vol.88, p.159-169
• Main Authors: Brandão-Costa, Romero M.P., Pajeu Nascimento, Thiago, Pedrosa Bezerra, Raquel, Porto, Ana L.F.
• Format: Article
• Language: English
• Subjects: Antifungal activity; Antioxidant; Biological assays; Cytotoxicity; Erythrocytes; Filicium decipiens; Fungicides; Gel filtration; Hemolysis; Ion exchange; Metabolites; Purification; Saponin; Saponins; Secondary metabolites; Seeds; Toxicity
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2019.10.018

[Display omitted] •Biological implications were attributed to FDS (FeliciumdecipiensSaponin).•Saponins isolated from several plants possess a broad spectrum of biological activities.•12.8 μg/mL of FDS caused more than 99.9% Aspergillus cell death within 24 h.•The hemolytic activity inhibition of FDS was correlated to the Lectin concentration. Filicium decipiens saponin (FDS) is the first saponin purified from F. decipiens seeds using ion-exchange and gel filtration chromatographies. In the present study, FDS and the aqueous crude extract of F. decipiens seeds were examined for their antifungal properties, hemolytic/cytotoxicity activity. The dried seeds were powdered and homogenized in 10% (w/v) for the preparation of crude extract. Sample obtained from Sephadex-LH-20 demonstrated FDS with a peak at 752.35 g/mol. FDS showed the highest toxicity against Aspergillus flavus, presenting with a low fungicidal concentration at 12.8 μg/mL. Under hemolytic induction, rabbit and cow erythrocytes were more affected by both samples, and the inhibition effect of PpyLL lectin was observed. These characteristics provide fundamental understanding of F. decipiens secondary metabolites, which would benefit future research to prevent dangerous traditional uses and provide agricultural solution.