Genotoxicity analysis of rutile titanium dioxide nanoparticles in mice after 28 days of repeated oral administration

Titanium dioxide (TiO 2 ) or titania has demonstrated excellent potential for commercial use in various arenas, such as in the paint, in pharmaceuticals and food industry. However information on the genotoxic potential of rutile form of TiO 2 -NP after repeated (28 days) low dose oral exposure in ma...

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Bibliographic Details
Published in:Nucleus (Calcutta) 2020-04, Vol.63 (1), p.17-24
Main Authors: Manivannan, J., Banerjee, Ritesh, Mukherjee, Anita
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Body weight
Bone marrow
Cell Biology
Clastogenicity
Comet assay
Cytotoxicity
DNA damage
Food industry
Genotoxicity
Hepatocytes
Life Sciences
Lymph nodes
Nanoparticles
Oral administration
Original Article
Reticuloendothelial system
Spleen
Titanium dioxide
Transmission electron microscopy
Zeta potential
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Summary:Titanium dioxide (TiO 2 ) or titania has demonstrated excellent potential for commercial use in various arenas, such as in the paint, in pharmaceuticals and food industry. However information on the genotoxic potential of rutile form of TiO 2 -NP after repeated (28 days) low dose oral exposure in major organs of the reticuloendothelial system (liver, spleen, bone marrow, lymph nodes) is not known. In this study Swiss albino male mice were gavaged TiO 2 -NP at sub-acute concentration (0.2, 0.4 and 0.8 mg/kg body weight) over a period of 28 days. Results revealed that TiO 2 -NP administered was of rutile form with mean average size of 25 nm by transmission electron microscopy. The values of PDI and Zeta potential from DLS of TiO 2 -NP in suspension specified that the nanomaterial was stable without much agglomeration. Chromosomal aberration assay showed that TiO 2 -NP was genotoxic and cytotoxic. DNA damage evaluation by comet assay confirmed that long term exposure to TiO 2 -NP at low concentrations can induce genotoxicity systemically in organs, such as liver, spleen, and thymus cells. Structural chromosomal aberration test from bone marrow cells revealed the clastogenicity of TiO 2 -NP at sub chronic low concentrations. Further in vivo studies are needed to elucidate the underlying mechanisms at the molecular level.
ISSN:0029-568X
0976-7975
DOI:10.1007/s13237-019-00277-0