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Set of Enzymatic Bioassays for Assessment of Soil Contamination
A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescenc...
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| Published in: | Doklady. Biological sciences 2019-11, Vol.489 (1), p.165-168 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c3304-267aa40f4521a117c2c2ba95026e4f7e8dafcd07f672823eec135d8c12d74c5b3 |
| container_end_page | 168 |
| container_issue | 1 |
| container_start_page | 165 |
| container_title | Doklady. Biological sciences |
| container_volume | 489 |
| creator | Kolosova, E. M. Sutormin, O. S. Esimbekova, E. N. Lonshakova-Mukina, V. I. Kratasyuk, V. A. |
| description | A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescence, respiration, etc. These functions are taken as test functions in classical bioassays with the use of living objects (luminous bacteria, daphnia, algae, and others). The regularities of the impact of different classes of toxicants on the activity of particular enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects: markers of contamination. Three enzyme systems with the maximal sensitivity to different classes of toxicants have been chosen for the set of enzymatic bioassays: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase, and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. The possibility to use enzymes instead of living organisms in the bioassay of natural complex systems has been shown. |
| doi_str_mv | 10.1134/S0012496619060024 |
| format | article |
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M. ; Sutormin, O. S. ; Esimbekova, E. N. ; Lonshakova-Mukina, V. I. ; Kratasyuk, V. A.</creator><creatorcontrib>Kolosova, E. M. ; Sutormin, O. S. ; Esimbekova, E. N. ; Lonshakova-Mukina, V. I. ; Kratasyuk, V. A.</creatorcontrib><description>A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescence, respiration, etc. These functions are taken as test functions in classical bioassays with the use of living objects (luminous bacteria, daphnia, algae, and others). The regularities of the impact of different classes of toxicants on the activity of particular enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects: markers of contamination. Three enzyme systems with the maximal sensitivity to different classes of toxicants have been chosen for the set of enzymatic bioassays: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase, and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. 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N.</creatorcontrib><creatorcontrib>Lonshakova-Mukina, V. I.</creatorcontrib><creatorcontrib>Kratasyuk, V. A.</creatorcontrib><title>Set of Enzymatic Bioassays for Assessment of Soil Contamination</title><title>Doklady. Biological sciences</title><addtitle>Dokl Biol Sci</addtitle><addtitle>Dokl Biol Sci</addtitle><description>A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescence, respiration, etc. These functions are taken as test functions in classical bioassays with the use of living objects (luminous bacteria, daphnia, algae, and others). The regularities of the impact of different classes of toxicants on the activity of particular enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects: markers of contamination. Three enzyme systems with the maximal sensitivity to different classes of toxicants have been chosen for the set of enzymatic bioassays: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase, and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. The possibility to use enzymes instead of living organisms in the bioassay of natural complex systems has been shown.</description><subject>Algae</subject><subject>Bioassays</subject><subject>Biomedical and Life Sciences</subject><subject>Contamination</subject><subject>Copper - analysis</subject><subject>Environmental Monitoring - methods</subject><subject>Enzyme Assays - methods</subject><subject>Enzymes</subject><subject>Evolutionary Biology</subject><subject>General Biology</subject><subject>L-Lactate dehydrogenase</subject><subject>Lactic acid</subject><subject>Life Sciences</subject><subject>Luminous bacteria</subject><subject>NAD</subject><subject>Oxidoreductase</subject><subject>Pesticides - analysis</subject><subject>Plant Sciences</subject><subject>Soil - chemistry</subject><subject>Soil contamination</subject><subject>Soil pollution</subject><subject>Titanium - analysis</subject><subject>Toxicants</subject><issn>0012-4966</issn><issn>1608-3105</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLAzEUhYMotlZ_gBsZcD16b54zK9FSH1BwUV0PaSaRKZ1JTaaL-utNbdWFuLpcznfOgUPIOcIVIuPXMwCkvJQSS5AAlB-QIUoocoYgDslwK-dbfUBOYlxA-kGxYzJgFBmIgg3Jzcz2mXfZpPvYtLpvTHbXeB2j3sTM-ZDdxmhjbG33Rc18s8zGvut123SJ9t0pOXJ6Ge3Z_o7I6_3kZfyYT58fnsa309wwBjynUmnNwXFBUSMqQw2d61IAlZY7ZYtaO1ODclLRgjJrDTJRFwZprbgRczYil7vcVfDvaxv7auHXoUuVFWUqJVIhy0ThjjLBxxisq1ahaXXYVAjVdrLqz2TJc7FPXs9bW_84vjdKAN0BMUndmw2_1f-nfgJbLXO0</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Kolosova, E. 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| language | eng |
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| subjects | Algae Bioassays Biomedical and Life Sciences Contamination Copper - analysis Environmental Monitoring - methods Enzyme Assays - methods Enzymes Evolutionary Biology General Biology L-Lactate dehydrogenase Lactic acid Life Sciences Luminous bacteria NAD Oxidoreductase Pesticides - analysis Plant Sciences Soil - chemistry Soil contamination Soil pollution Titanium - analysis Toxicants |
| title | Set of Enzymatic Bioassays for Assessment of Soil Contamination |
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