Comparisons of Spore Dosimetry and Spectral Photometry of Solar-UV Radiation at Four Sites in Japan and Europe

• Published in: Photochemistry and photobiology 2000-12, Vol.72 (6), p.739-745
• Main Authors: Munakata, Nobuo, Kazadzis, Stelios, Bais, Alkiviadis F., Hieda, Kotaro, Rontó, Györgyi, Rettberg, Petra, Horneck, Gerda
• Format: Article
• Language: English
• Subjects: Bacillus subtilis - growth & development; Bacillus subtilis - radiation effects; Dose-Response Relationship, Radiation; ENVIRONMENTAL PHOTOBIOLOGY AND UVR EFFECTS; Europe; Japan; Spores, Bacterial; Sunlight
• ISSN: 0031-8655; 1751-1097
• DOI: 10.1562/0031-8655(2000)072<0739:COSDAS>2.0.CO;2

In order to develop monitoring and assessment systems of biologically effective doses of solar-UV radiation, concurrent measurements of spectral photometry and spore dosimetry were conducted in summer months at four sites in Japan and Europe. Effectiveness spectra were derived by multiplying spectral irradiance in 0.5 nm steps between 290 and 400 nm with the inactivation efficiency of the spores determined using monochromatic radiation of fine wavelength resolution. Shapes of the effectiveness spectra were very similar at the four sites exhibiting major peaks at 303.5, 305.0, 307.5 and 311.0 nm. The dose rates for spore inactivation from direct survival measurements and from calculations by the integration of the effectiveness spectra were compared for 174 data points. The ratios (observed/calculated) of the two values were concordant with a mean of 1.26 (±0.24 standard deviation [SD]). The possible causes for the variations and slightly larger observed values are discussed.