Photodynamic Effect of Iron Excess on Photosystem II Function in Pea Plants

• Published in: Photochemistry and photobiology 2002-05, Vol.75 (5), p.513-518
• Main Authors: Suh, Hwa-Jin, Sook Kim, Chang, Lee, Ji-Yeun, Jung, Jin
• Format: Article
• Language: English
• Subjects: Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Iron - pharmacology; Light; PHOTOSYNTHESIS AND BIO/CHEMILUMINESCENCE; Photosynthetic Reaction Center Complex Proteins - drug effects; Photosynthetic Reaction Center Complex Proteins - metabolism; Photosynthetic Reaction Center Complex Proteins - radiation effects; Photosystem II Protein Complex; Pisum sativum - drug effects; Pisum sativum - metabolism; Pisum sativum - radiation effects
• ISSN: 0031-8655; 1751-1097
• DOI: 10.1562/0031-8655(2002)075<0513:PEOIEO>2.0.CO;2

An earlier mechanistic phase of iron toxicity in photosynthetic cells was interpreted in terms of enhanced photodynamic action by the cytochrome b6/f complex (Cyt b6/f) via singlet oxygen (1O2) on the photosystem II complex (PS II). Iron excess was induced in hydroponically cultured pea (Pisum sativum L.) plants, and its effect on the function of PS II in vivo as well as in vitro was studied under high-irradiance conditions. Iron excess in plants gave rise to a significant increase in Cyt b6/f content of thylakoids. It appeared that the larger the content of Cyt b6/f, the more susceptible PS II was to photoinhibition, and the higher the rate of 1O2 photoproduction in thylakoids was. The action spectrum for degradation of the D1 protein in thylakoids revealed that photosensitization by nonporphyrin chromophore(s) was apparently associated with near UV to blue light–induced deterioration of PS II. The results are pertinent to the concept that photooxidative damage to PS II, exacerbated by iron accumulation in thylakoid membranes in the form of Cyt b6/f, is involved in the mechanism of iron toxicity in leaf cells.