Effects of the Interaction Between ß-Carboline-3-carboxylic acid N-Methylamide and Polynucleotides on Singlet Oxygen Quantum Yield and DNA Oxidative Damage

The complexation of β-carboline-3-carboxylic acid N-methylamide (βCMAM) with the sodium salts of the nucleotides polyadenylic (Poly A), polycytidylic (Poly C), polyguanylic (Poly G), polythymidylic (Poly T) and polyuridylic (Poly U) acids, and with double stranded (dsDNA) and single stranded deoxyri...

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Bibliographic Details
Published in:Photochemistry and photobiology 2007-11, Vol.83 (6), p.1455
Main Authors: García-Zubiri, Iñigo X, Burrows, Hugh D, Melo, J Sérgio Seixas de, Pina, Joao, Monteserín, María, Tapia, María J
Format: Article
Language:English
Subjects:
Apoptosis
Bond strength
DNA damage
Light therapy
Oxygen
Photodynamic therapy
Sulfuric acid
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Summary:The complexation of β-carboline-3-carboxylic acid N-methylamide (βCMAM) with the sodium salts of the nucleotides polyadenylic (Poly A), polycytidylic (Poly C), polyguanylic (Poly G), polythymidylic (Poly T) and polyuridylic (Poly U) acids, and with double stranded (dsDNA) and single stranded deoxyribonucleic acids (ssDNA) was studied at pH 4, 6 and 9. Predominant 1:1 complex formation is indicated from Job plots. Association constants were determined using the Benesi-Hildebrand equation. βCMAM-sensitized singlet oxygen quantum yields were determined at pH 4, 6 and 9, and the effects on this of adding oligonucleotides, dsDNA and ssDNA were studied at the three pH values. With dsDNA, the effect on βCMAM triplet state formation was also determined through triplet-triplet transient absorption spectra. To evaluate possible oxidative damage of DNA following singlet oxygen βCMAM photosensitization, we used thiobarbituric acid-reactivity assays and electrophoretic separation of DNA assays. The results showed no oxidative damage at the level of DNA degradation or strand break. [PUBLICATION ABSTRACT]
ISSN:0031-8655
1751-1097