Ultrasonication of Milky Stage Rice Milk with Bioactive Peptides from Rice Bran: Its Bioactivities and Absorption

• Published in: Food and bioprocess technology 2020-03, Vol.13 (3), p.462-474
• Main Authors: Ngamsuk, Samuchaya, Hsu, Jue-Liang, Huang, Tzou-Chi, Suwannaporn, Prisana
• Format: Article
• Language: English
• Subjects: Absorption; Agriculture; Antioxidants; Beverages; Bioactive compounds; Biological activity; Biotechnology; Cell size; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Food Science; Functional foods & nutraceuticals; High-performance liquid chromatography; Intestinal absorption; Intestine; Liquid chromatography; Milk; Original Paper; Peptides; Plant-based beverages; Proteins; Rice; Rice bran; Sonication; Subtilisin; Trypsin
• ISSN: 1935-5130; 1935-5149
• DOI: 10.1007/s11947-019-02371-2

Rice in early development stage contains peptides and various bioactive compounds. Rice protein hydrolysate was prepared from Riceberry bran protein hydrolysed with Alcalase® and trypsin. Protein hydrolysate from Alcalase® (MW < 3 kDa) was fractionated into 12 fractions using RP-HPLC and tested for antioxidant, ACE, and ROS-inhibitory activity. Peptide from RP-HPLC fraction 1 showed that the lowest ROS inhibition and highest antioxidant were identified by LC-MS/MS as Val-Leu-Ala-Ala-Gly-Cys-Pro-Leu. Peptide fraction 4 showed that the strongest in vitro ACE-inhibitory activity was identified as Ala-Met-Ser-Phe-Ala-Glu-Met. Young Riceberry milk was then enriched with Alcalase® protein hydrolysate (MW < 3 kDa) and sonicated at amplitude 20% for 30 min and 40% for 60 min. Particle size, transmittance, and Caco-2 cell absorption were measured. The highest peptide content and % absorption was found in young rice milk ultrasonicated at 40% for 60 min. Rice bran protein hydrolysate produced by Alcalase® had much smaller MW bioactive peptides and could be used as a potent functional food ingredient. The young rice milk fortified with bioactive peptides after high power ultra-sonication could increase the in vitro intestinal absorption.