Optimization of conditions for the induction of Tagetes patula L. hairy roots

The hairy root cultures are promising sources of secondary metabolites of plants, including rare and endangered species. They possess genetic and biochemical stability, unlimited growth rate in free-hormone medium, short doubling times, high biosynthetic activity and ecological purity of plant raw m...

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Bibliographic Details
Published in:Ukrainian journal of ecology 2019-10, Vol.9 (3), p.415-420
Main Authors: Khlebova, L. P., Brovko, E. S., Bychkova, O. V., Pavlova, N. V.
Format: Article
Language:English
Subjects:
Bacteria
Biocides
Biological activity
Clones
Cultures
Ecology
Endangered & extinct species
Endangered plants
Endangered species
Explants
Genetic transformation
Growth rate
Hairy root
Hormones
Leaves
Metabolites
Rare species
Raw materials
Roots
Scaling
Secondary metabolites
Seeds
Stability
Tagetes patula
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Summary:The hairy root cultures are promising sources of secondary metabolites of plants, including rare and endangered species. They possess genetic and biochemical stability, unlimited growth rate in free-hormone medium, short doubling times, high biosynthetic activity and ecological purity of plant raw materials. The hairy root cultures of Tagetes patula L. can be used to produce biologically active substances with biocidal activity. The study aimed to determine the virulent strain of Agrobacterium rhizogenes and the most effective period of co-cultivation of T. patula leaf explants with an agrobacterium to induce actively growing hairy root cultures. We used 3 strains (A-4b, 8196RT and 15834). The time of infection ranged from 3 to 33 hours in increments of 3 hours. We found that 24 h is the best time of infection to induce hairy roots with the highest transformation efficiency (92%). The wild strain A. rhizogenes 15834 turned out to be the most virulent when infected leaf explants of spreading marigold. This strain provided the maximum transformation effect, reaching 85.4%. We have identified 5 actively growing clones of hairy roots with intensive branching, the growth indices of which were 64-75. In the future, they will be transferred to a liquid medium for biomass accumulation and scaling.
ISSN:2520-2138
2520-2138
DOI:10.15421/2019_119