Rapid screening and quantitative detection of Salmonella using a quantum dot nanobead-based biosensor

The continuing hurdle of developing foodborne pathogen detection techniques is that compromises must be made among simplicity, portability, speed, sensitivity, and quantitation. Herein, we fabricated quantum dot nanobeads (QDNS) by a layer-by-layer assembly of quantum dots on the surface of polymer...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2020-03, Vol.145 (6), p.2184-2190
Main Authors: Hu, Jiao, Tang, Feng, Jiang, Yong-Zhong, Liu, Cui
Format: Article
Language:English
Subjects:
Accuracy
Acrylic Resins - chemistry
Antibodies, Immobilized - immunology
Biosensing Techniques - methods
Biosensors
Cadmium - chemistry
Fluorescence
Immunoassay
Immunoassay - methods
Limit of Detection
Nanospheres
Nanospheres - chemistry
Polystyrenes - chemistry
Quantum dots
Quantum Dots - chemistry
Reproducibility of Results
Salmonella
Salmonella typhimurium - immunology
Salmonella typhimurium - isolation & purification
Screening
Selenium - chemistry
Sensitivity
Single-Blind Method
Sulfides - chemistry
Zinc Compounds - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The continuing hurdle of developing foodborne pathogen detection techniques is that compromises must be made among simplicity, portability, speed, sensitivity, and quantitation. Herein, we fabricated quantum dot nanobeads (QDNS) by a layer-by-layer assembly of quantum dots on the surface of polymer nanospheres. QDNS exhibited higher fluorescence intensity than the quantum dots at the same particle number. Based on the quantum dot nanobeads as the signal reporter, a quantitative lateral flow immunoassay was demonstrated for Salmonella typhimurium detection with improved sensitivity, specificity and accuracy. A visual detection limit of 5 × 103 CFU mL-1Salmonella typhimurium within 10 min has been proved and demonstrated. Additionally, higher concentrations of non-Salmonella typhimurium bacteria have negligible effects on the detection of Salmonella typhimurium. The results of 50 single blind tests by 10 testers suggested that the assay exhibited 100% accuracy. The results illustrate that the assay provides a balance among simplicity, speed, sensitivity and accuracy, and it can be a favorable alternative for Salmonella typhimurium screening in various samples.
ISSN:0003-2654
1364-5528
DOI:10.1039/d0an00035c