Development and Validation of a New Derivative UV Spectrophotometric Method for Simultaneous Quantification of Tizanidine and Aceclofenac in Tablets

[...]an attempt has been made to develop and validate a simple derivative UV spectroscopic method for simultaneous estimation of these drugs in combination dosage forms. The differentiation of zero-order spectrum can lead to separation of overlapped signals, elimination of background caused by prese...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2020-02, Vol.13 (2), p.569-574
Main Authors: Sowjanya, G., Pallavi, D. Parimala, Mazumder, Oindrila, Kumar, S. Praveen
Format: Article
Language:English
Subjects:
Accuracy
Calibration
Caustic soda
Drug dosages
Methods
Spasticity
Standard deviation
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Summary:[...]an attempt has been made to develop and validate a simple derivative UV spectroscopic method for simultaneous estimation of these drugs in combination dosage forms. The differentiation of zero-order spectrum can lead to separation of overlapped signals, elimination of background caused by presence of other compounds in a sample, improvement of resolution of mixtures as it enhances the detectability of minor spectral features, and enhancement of sensitivity and specificity. From the zero order spectra of Tizanidine and Aceclofenac obtained in the UV range of 200-400nm, the spectra were derivatized using the inbuilt software into first order spectra for which the derivative absorbance (at minima) was calculated at the corresponding zero crossing points and calibration curves were plotted. Where, σ is the standard deviation of the absorbance of the sample S is the slope of the related calibration graphs Assay: The developed method was also applied for quantification of Tizanidine and Aceclofenac in marketed dosage forms.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.5958/0974-360X.2020.00107.9