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Assembly and shifts of the bacterial rhizobiome of field grown transgenic maize line carrying mcry1Ab and mcry2Ab genes at different developmental stages
Assessing the effects of transgenic crops on rhizobacterial communities has catalyzed numerous studies in the recent past decades. To address further to this concern, we designed research to examine the variations in the bacterial diversity profiles of transgenic insect-resistant maize line 2A-5 (2A...
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| Published in: | Plant growth regulation 2020-05, Vol.91 (1), p.113-126 |
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| container_title | Plant growth regulation |
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| creator | Fazal, Aliya Wen, Zhong-Ling Lu, Yun-Ting Hua, Xiao-Mei Yang, Min-Kai Yin, Tong-Ming Han, Hong-Wei Lin, Hong-Yan Wang, Xiao-Ming Lu, Gui-Hua Qi, Jin-Liang Yang, Yong-Hua |
| description | Assessing the effects of transgenic crops on rhizobacterial communities has catalyzed numerous studies in the recent past decades. To address further to this concern, we designed research to examine the variations in the bacterial diversity profiles of transgenic insect-resistant maize line 2A-5 (2A5) carrying
mcry1Ab
and
mcry2Ab
genes and its control Zheng58 (Z58), at different developmental stages under natural field conditions. High-throughput sequencing of the 16S rDNA gene (V3–V4) hyper-variable region via Illumina MiSeq revealed no significant shifts in the overall α and β-diversity. However, some developmental stage-dependent discrepancies were observed in the relative abundances of microbial communities. At the jointing stage, members of the phylum Proteobacteria, and the genera
Rahnella
,
Kaistobacter
,
Pseudomonas
and
Arthrobacter
were greatly enriched in the rhizosphere soil of 2A5 and Z58 compared to bulk, and surrounding soil while de-riched with the microbes belonging to the genera
Bacillus
and
Flavobacterium
. In addition, the relative abundances of nitrogen fixing microbes i.e.
Arthrobacter
,
Burkholderia
and
Pseudomonas
in the rhizosphere samples of 2A5 showed exuberance compared to those of Z58 at the flowering and maturation stages, implying that genetic modification might play a role in the enrichment/derichment of some nitrogen fixing microbes. The results of our study indicate little to no impacts of transgenic maize carrying
mcry1Ab
and
mcry2Ab
genes and also, the changes in the rhizosphere bacterial communities are imposed by host plant during different developmental stages. |
| doi_str_mv | 10.1007/s10725-020-00591-7 |
| format | article |
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mcry1Ab
and
mcry2Ab
genes and its control Zheng58 (Z58), at different developmental stages under natural field conditions. High-throughput sequencing of the 16S rDNA gene (V3–V4) hyper-variable region via Illumina MiSeq revealed no significant shifts in the overall α and β-diversity. However, some developmental stage-dependent discrepancies were observed in the relative abundances of microbial communities. At the jointing stage, members of the phylum Proteobacteria, and the genera
Rahnella
,
Kaistobacter
,
Pseudomonas
and
Arthrobacter
were greatly enriched in the rhizosphere soil of 2A5 and Z58 compared to bulk, and surrounding soil while de-riched with the microbes belonging to the genera
Bacillus
and
Flavobacterium
. In addition, the relative abundances of nitrogen fixing microbes i.e.
Arthrobacter
,
Burkholderia
and
Pseudomonas
in the rhizosphere samples of 2A5 showed exuberance compared to those of Z58 at the flowering and maturation stages, implying that genetic modification might play a role in the enrichment/derichment of some nitrogen fixing microbes. The results of our study indicate little to no impacts of transgenic maize carrying
mcry1Ab
and
mcry2Ab
genes and also, the changes in the rhizosphere bacterial communities are imposed by host plant during different developmental stages.</description><identifier>ISSN: 0167-6903</identifier><identifier>EISSN: 1573-5087</identifier><identifier>DOI: 10.1007/s10725-020-00591-7</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Arthrobacter ; Bacteria ; Biomedical and Life Sciences ; Corn ; Crops ; Developmental stages ; Experiments ; Flowering ; Gene expression ; Genes ; Genetic modification ; Genetically altered foods ; Host plants ; Insects ; Jointing ; Life Sciences ; Microbial activity ; Microorganisms ; Next-generation sequencing ; Nitrogen ; Nitrogen fixation ; Nitrogenation ; Original Paper ; Plant Anatomy/Development ; Plant Physiology ; Plant Sciences ; Pseudomonas ; Rhizosphere ; Rice ; rRNA 16S ; Soils ; Transgenic plants ; Variable region</subject><ispartof>Plant growth regulation, 2020-05, Vol.91 (1), p.113-126</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Plant Growth Regulation is a copyright of Springer, (2020). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-d8b7308b2eb8256f85e6d0f6f43edef59ebc02cfcf8e22117916760f6dca13d23</citedby><cites>FETCH-LOGICAL-c319t-d8b7308b2eb8256f85e6d0f6f43edef59ebc02cfcf8e22117916760f6dca13d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids></links><search><creatorcontrib>Fazal, Aliya</creatorcontrib><creatorcontrib>Wen, Zhong-Ling</creatorcontrib><creatorcontrib>Lu, Yun-Ting</creatorcontrib><creatorcontrib>Hua, Xiao-Mei</creatorcontrib><creatorcontrib>Yang, Min-Kai</creatorcontrib><creatorcontrib>Yin, Tong-Ming</creatorcontrib><creatorcontrib>Han, Hong-Wei</creatorcontrib><creatorcontrib>Lin, Hong-Yan</creatorcontrib><creatorcontrib>Wang, Xiao-Ming</creatorcontrib><creatorcontrib>Lu, Gui-Hua</creatorcontrib><creatorcontrib>Qi, Jin-Liang</creatorcontrib><creatorcontrib>Yang, Yong-Hua</creatorcontrib><title>Assembly and shifts of the bacterial rhizobiome of field grown transgenic maize line carrying mcry1Ab and mcry2Ab genes at different developmental stages</title><title>Plant growth regulation</title><addtitle>Plant Growth Regul</addtitle><description>Assessing the effects of transgenic crops on rhizobacterial communities has catalyzed numerous studies in the recent past decades. To address further to this concern, we designed research to examine the variations in the bacterial diversity profiles of transgenic insect-resistant maize line 2A-5 (2A5) carrying
mcry1Ab
and
mcry2Ab
genes and its control Zheng58 (Z58), at different developmental stages under natural field conditions. High-throughput sequencing of the 16S rDNA gene (V3–V4) hyper-variable region via Illumina MiSeq revealed no significant shifts in the overall α and β-diversity. However, some developmental stage-dependent discrepancies were observed in the relative abundances of microbial communities. At the jointing stage, members of the phylum Proteobacteria, and the genera
Rahnella
,
Kaistobacter
,
Pseudomonas
and
Arthrobacter
were greatly enriched in the rhizosphere soil of 2A5 and Z58 compared to bulk, and surrounding soil while de-riched with the microbes belonging to the genera
Bacillus
and
Flavobacterium
. In addition, the relative abundances of nitrogen fixing microbes i.e.
Arthrobacter
,
Burkholderia
and
Pseudomonas
in the rhizosphere samples of 2A5 showed exuberance compared to those of Z58 at the flowering and maturation stages, implying that genetic modification might play a role in the enrichment/derichment of some nitrogen fixing microbes. The results of our study indicate little to no impacts of transgenic maize carrying
mcry1Ab
and
mcry2Ab
genes and also, the changes in the rhizosphere bacterial communities are imposed by host plant during different developmental stages.</description><subject>Agriculture</subject><subject>Arthrobacter</subject><subject>Bacteria</subject><subject>Biomedical and Life Sciences</subject><subject>Corn</subject><subject>Crops</subject><subject>Developmental stages</subject><subject>Experiments</subject><subject>Flowering</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic modification</subject><subject>Genetically altered foods</subject><subject>Host plants</subject><subject>Insects</subject><subject>Jointing</subject><subject>Life Sciences</subject><subject>Microbial activity</subject><subject>Microorganisms</subject><subject>Next-generation sequencing</subject><subject>Nitrogen</subject><subject>Nitrogen fixation</subject><subject>Nitrogenation</subject><subject>Original Paper</subject><subject>Plant Anatomy/Development</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Pseudomonas</subject><subject>Rhizosphere</subject><subject>Rice</subject><subject>rRNA 16S</subject><subject>Soils</subject><subject>Transgenic plants</subject><subject>Variable region</subject><issn>0167-6903</issn><issn>1573-5087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc1KxDAUhYMoOP68gKuA6-pNMm3S5SD-geBG1yFNbzoZ2nRMqjK-iW9rZkZw5-oeuN85F-4h5ILBFQOQ14mB5GUBHAqAsmaFPCAzVkpRlKDkIZkBq2RR1SCOyUlKKwBQqmQz8r1ICYem31ATWpqW3k2Jjo5OS6SNsRNGb3oal_5rbPw44HbnPPYt7eL4GegUTUgdBm_pYPwX0t4HpNbEuPGho4ONG7ZoduFbzbPONCZqJtp65zBiyAo_sB_XQ9b5WppMh-mMHDnTJzz_nafk9e725eaheHq-f7xZPBVWsHoqWtVIAarh2CheVk6VWLXgKjcX2KIra2wscOusU8g5Y7LOn6gy0FrDRMvFKbnc567j-PaOadKr8T2GfFJzoeZVzfMbM8X3lI1jShGdXkc_mLjRDPS2Ar2vQOcK9K4CLbNJ7E0pw6HD-Bf9j-sH2SeMoQ</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Fazal, Aliya</creator><creator>Wen, Zhong-Ling</creator><creator>Lu, Yun-Ting</creator><creator>Hua, Xiao-Mei</creator><creator>Yang, Min-Kai</creator><creator>Yin, Tong-Ming</creator><creator>Han, Hong-Wei</creator><creator>Lin, Hong-Yan</creator><creator>Wang, Xiao-Ming</creator><creator>Lu, Gui-Hua</creator><creator>Qi, Jin-Liang</creator><creator>Yang, Yong-Hua</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20200501</creationdate><title>Assembly and shifts of the bacterial rhizobiome of field grown transgenic maize line carrying mcry1Ab and mcry2Ab genes at different developmental stages</title><author>Fazal, Aliya ; Wen, Zhong-Ling ; Lu, Yun-Ting ; Hua, Xiao-Mei ; Yang, Min-Kai ; Yin, Tong-Ming ; Han, Hong-Wei ; Lin, Hong-Yan ; Wang, Xiao-Ming ; Lu, Gui-Hua ; Qi, Jin-Liang ; Yang, Yong-Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-d8b7308b2eb8256f85e6d0f6f43edef59ebc02cfcf8e22117916760f6dca13d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agriculture</topic><topic>Arthrobacter</topic><topic>Bacteria</topic><topic>Biomedical and Life Sciences</topic><topic>Corn</topic><topic>Crops</topic><topic>Developmental stages</topic><topic>Experiments</topic><topic>Flowering</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic modification</topic><topic>Genetically altered foods</topic><topic>Host plants</topic><topic>Insects</topic><topic>Jointing</topic><topic>Life Sciences</topic><topic>Microbial activity</topic><topic>Microorganisms</topic><topic>Next-generation sequencing</topic><topic>Nitrogen</topic><topic>Nitrogen fixation</topic><topic>Nitrogenation</topic><topic>Original Paper</topic><topic>Plant Anatomy/Development</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Pseudomonas</topic><topic>Rhizosphere</topic><topic>Rice</topic><topic>rRNA 16S</topic><topic>Soils</topic><topic>Transgenic plants</topic><topic>Variable region</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fazal, Aliya</creatorcontrib><creatorcontrib>Wen, Zhong-Ling</creatorcontrib><creatorcontrib>Lu, Yun-Ting</creatorcontrib><creatorcontrib>Hua, Xiao-Mei</creatorcontrib><creatorcontrib>Yang, Min-Kai</creatorcontrib><creatorcontrib>Yin, Tong-Ming</creatorcontrib><creatorcontrib>Han, Hong-Wei</creatorcontrib><creatorcontrib>Lin, Hong-Yan</creatorcontrib><creatorcontrib>Wang, Xiao-Ming</creatorcontrib><creatorcontrib>Lu, Gui-Hua</creatorcontrib><creatorcontrib>Qi, Jin-Liang</creatorcontrib><creatorcontrib>Yang, Yong-Hua</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>ProQuest research library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Plant growth regulation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fazal, Aliya</au><au>Wen, Zhong-Ling</au><au>Lu, Yun-Ting</au><au>Hua, Xiao-Mei</au><au>Yang, Min-Kai</au><au>Yin, Tong-Ming</au><au>Han, Hong-Wei</au><au>Lin, Hong-Yan</au><au>Wang, Xiao-Ming</au><au>Lu, Gui-Hua</au><au>Qi, Jin-Liang</au><au>Yang, Yong-Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assembly and shifts of the bacterial rhizobiome of field grown transgenic maize line carrying mcry1Ab and mcry2Ab genes at different developmental stages</atitle><jtitle>Plant growth regulation</jtitle><stitle>Plant Growth Regul</stitle><date>2020-05-01</date><risdate>2020</risdate><volume>91</volume><issue>1</issue><spage>113</spage><epage>126</epage><pages>113-126</pages><issn>0167-6903</issn><eissn>1573-5087</eissn><abstract>Assessing the effects of transgenic crops on rhizobacterial communities has catalyzed numerous studies in the recent past decades. To address further to this concern, we designed research to examine the variations in the bacterial diversity profiles of transgenic insect-resistant maize line 2A-5 (2A5) carrying
mcry1Ab
and
mcry2Ab
genes and its control Zheng58 (Z58), at different developmental stages under natural field conditions. High-throughput sequencing of the 16S rDNA gene (V3–V4) hyper-variable region via Illumina MiSeq revealed no significant shifts in the overall α and β-diversity. However, some developmental stage-dependent discrepancies were observed in the relative abundances of microbial communities. At the jointing stage, members of the phylum Proteobacteria, and the genera
Rahnella
,
Kaistobacter
,
Pseudomonas
and
Arthrobacter
were greatly enriched in the rhizosphere soil of 2A5 and Z58 compared to bulk, and surrounding soil while de-riched with the microbes belonging to the genera
Bacillus
and
Flavobacterium
. In addition, the relative abundances of nitrogen fixing microbes i.e.
Arthrobacter
,
Burkholderia
and
Pseudomonas
in the rhizosphere samples of 2A5 showed exuberance compared to those of Z58 at the flowering and maturation stages, implying that genetic modification might play a role in the enrichment/derichment of some nitrogen fixing microbes. The results of our study indicate little to no impacts of transgenic maize carrying
mcry1Ab
and
mcry2Ab
genes and also, the changes in the rhizosphere bacterial communities are imposed by host plant during different developmental stages.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10725-020-00591-7</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-6903 |
| ispartof | Plant growth regulation, 2020-05, Vol.91 (1), p.113-126 |
| issn | 0167-6903 1573-5087 |
| language | eng |
| recordid | cdi_proquest_journals_2384692573 |
| source | Springer Nature |
| subjects | Agriculture Arthrobacter Bacteria Biomedical and Life Sciences Corn Crops Developmental stages Experiments Flowering Gene expression Genes Genetic modification Genetically altered foods Host plants Insects Jointing Life Sciences Microbial activity Microorganisms Next-generation sequencing Nitrogen Nitrogen fixation Nitrogenation Original Paper Plant Anatomy/Development Plant Physiology Plant Sciences Pseudomonas Rhizosphere Rice rRNA 16S Soils Transgenic plants Variable region |
| title | Assembly and shifts of the bacterial rhizobiome of field grown transgenic maize line carrying mcry1Ab and mcry2Ab genes at different developmental stages |
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