Rapid, Label‐free Optical Spectroscopy Platform for Diagnosis of Heparin‐Induced Thrombocytopenia

The use of surface‐enhanced Raman spectroscopy (SERS) to determine spectral markers for the diagnosis of heparin‐induced thrombocytopenia (HIT), a difficult‐to‐diagnose immune‐related complication that often leads to limb ischemia and thromboembolism, is proposed. The ability to produce distinct mol...

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Bibliographic Details
Published in:Angewandte Chemie 2020-04, Vol.132 (15), p.6028-6034
Main Authors: Huang, Zufang, Siddhanta, Soumik, Zheng, Gang, Kickler, Thomas, Barman, Ishan
Format: Article
Language:English
Subjects:
Analytische Methoden
Biosensoren
Capillary tubes
Chemistry
Diagnosis
Diagnostic software
Diagnostic systems
Heparin
Heparin-induzierte Thrombozytopenie
Ischemia
Learning algorithms
Machine learning
Nanopartikel
Raman spectroscopy
Raman-Spektroskopie
Spectroscopy
Spectrum analysis
Thrombocytopenia
Thromboembolism
Workflow
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Summary:The use of surface‐enhanced Raman spectroscopy (SERS) to determine spectral markers for the diagnosis of heparin‐induced thrombocytopenia (HIT), a difficult‐to‐diagnose immune‐related complication that often leads to limb ischemia and thromboembolism, is proposed. The ability to produce distinct molecular signatures without the addition of labels enables unbiased inquiry and makes SERS an attractive complementary diagnostic tool. A capillary‐tube‐derived SERS platform offers ultrasensitive, label‐free measurement as well as efficient handling of blood serum samples. This shows excellent reproducibility, long‐term stability and provides an alternative diagnostic rubric for the determination of HIT by leveraging machine‐learning‐based classification of the spectroscopic data. We envision that a portable Raman instrument could be combined with the capillary‐tube‐based SERS analytical tool for diagnosis of HIT in the clinical laboratory, without perturbing the existing diagnostic workflow. HIT‐verdächtig? Die klinische Detektion einer Heparin‐induzierten Thrombozytopenie (HIT), einer durch eine Immunreaktion ausgelösten Komplikation der Heparintherapie, ist anspruchsvoll. Eine Kapillarröhrchen‐basierte SERS‐Plattform ermöglicht die korrekte Erkennung dieser Pathologie durch eine markerfreie, rasche Analyse des Patientenserums. SERS=Oberflächen‐verstärkte Raman‐Streuung.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201913970