Improved Method for n-Alkanes and n-Methyl-Ketones Determination in Litters and Soils

A new technique to extract lipid biomarkers ( n- alkanes and n- methyl-ketones) from forest litters and soils using the accelerated solvent extraction (ASE) method was developed. The maximum output of n- alkanes and n- methyl-ketones was obtained by sequential extraction using the chloroform : metha...

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Bibliographic Details
Published in:Eurasian soil science 2020-03, Vol.53 (3), p.310-316
Main Authors: Zavgorodnyaya, Yu. A., Anokhina, N. A., Bogatyrev, L. G., Demin, V. V.
Format: Article
Language:English
Subjects:
Alkanes
Biodegradation
Biomarkers
Chloroform
Cycles
Earth and Environmental Science
Earth Sciences
Geotechnical Engineering & Applied Earth Sciences
Ketones
Lipids
Methods
Soil
Soil Chemistry
Soils
Solvent extraction
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Summary:A new technique to extract lipid biomarkers ( n- alkanes and n- methyl-ketones) from forest litters and soils using the accelerated solvent extraction (ASE) method was developed. The maximum output of n- alkanes and n- methyl-ketones was obtained by sequential extraction using the chloroform : methanol mixture [3 : 1 (vol)] in the first two extraction cycles and chloroform in the subsequent two cycles. The extraction has been carried out at the temperature of 100°С, pressure of 10.3 MPa, and static extraction time of 5 min. The repeated chloroform treatment by ASE was shown to increase the output of long-chain n- alkanes and n- methyl-ketones.
ISSN:1064-2293
1556-195X
DOI:10.1134/S1064229320030114