The Twice-Oxidized Graphene Oxide/Gold Nanoparticles Composite SERS Substrate for Sensitive Detection of Clenbuterol Residues in Animal-Origin Food Samples

The illegal use of clenbuterol (CL), as a growth promoter in animal feeding, is banned in the majority of countries across the world, such as the European Union and China. In this study, a rapid and sensitive method for detecting CL in animal-origin food samples based on liquid-liquid extraction and...

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Bibliographic Details
Published in:Food analytical methods 2020-04, Vol.13 (4), p.902-910
Main Authors: Cheng, Jie, Fan, Mengdie, Wang, Peilong, Su, Xiao-Ou
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Clenbuterol
Environmental cleanup
Food
Food quality
Food safety
Food Science
Gold
Graphene
Liquid chromatography
Liquid-liquid extraction
Mass spectrometry
Mass spectroscopy
Microbiology
Nanoparticles
Quality control
Quantitation
Raman spectroscopy
Stability analysis
Substrates
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Summary:The illegal use of clenbuterol (CL), as a growth promoter in animal feeding, is banned in the majority of countries across the world, such as the European Union and China. In this study, a rapid and sensitive method for detecting CL in animal-origin food samples based on liquid-liquid extraction and surface-enhanced Raman spectroscopy (SERS) has been developed for the first time. A twice-oxidized graphene oxide/gold nanoparticles composite substrate was innovatively synthesized and used to enhance the characteristic Raman signal of CL at △ v  = 1474 cm − 1 and 1608 cm − 1 , and no more than 10 min of sample cleanup was required before SERS analysis. The optimized hybrid substrate demonstrated good stability over a period of 240 days, which is more stable than previous studies. Quantitation calculation was performed in the concentration range of 1–100 ng g − 1 with a limit of detection (LOD) as low as 1 ng g − 1 . Furthermore, the novel detection method was verified for use in the identification of real samples, and the results were consistent with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS). The simplicity and reliability of the proposed method demonstrate its great potential for practical applications in the field of quality control and food safety.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-020-01709-3