Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor

Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of o -phenylenediamine and ascorbic acid was develo...

Full description

Saved in:
Bibliographic Details
Published in:Food analytical methods 2020-04, Vol.13 (4), p.1008-1016
Main Authors: Zha, Yonghong, Liu, Xiaolei, Hu, Pan, Lu, Shiying, Ren, Honglin, Liu, Zengshan, Liang, Xiongyan, Yang, Yuying, Li, Yansong, Zhou, Yu
Format: Article
Language:English
Subjects:
Acetochlor
Alkaline phosphatase
Analytical Chemistry
Ascorbic acid
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Corn
Enzyme-linked immunosorbent assay
Fluorescence
Food Science
Herbicides
Immunoassay
Metolachlor
Microbiology
o-Phenylenediamine
Pesticides
Phenylenediamine
Phosphatase
Sensitivity
Sensitivity analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of o -phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. The new immunoassay revealed limits of detection (LODs) for acetochlor (ATC), metolachlor, and propisochlor of 1.19 ng/mL, 2.34 ng/mL, and 3.40 ng/mL, respectively. The average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. Such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-020-01706-6